通过核糖体RNA基因的限制性片段长度多态性对偶然分枝杆菌-龟分枝杆菌复合群进行快速鉴定

Rapid characterization of Mycobacterium fortuitum-chelonei complex by restriction fragment length polymorphism of ribosomal RNA genes.

作者信息

Kanaujia G V, Katoch V M, Shivannavar C T, Sharma V D, Patil M A

机构信息

Central JALMA Institute for Leprosy (ICMR), Agra, India.

出版信息

FEMS Microbiol Lett. 1991 Jan 15;61(2-3):205-8. doi: 10.1016/0378-1097(91)90552-l.

DOI:10.1016/0378-1097(91)90552-l

PMID:1674710

Abstract

Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose membrane. Fragments of DNA containing rRNA genes were identified by hybridization with gamma-32P-labelled rRNA. Patterns were found to be species specific and both the species were distinguishable from each other. Results indicate that this approach can be used for rapid genomic characterization of the Mycobacterium fortuitum-chelonei complex.

摘要

以标记的γ-32P分枝杆菌核糖体RNA（rRNA）为探针，分析了偶然分枝杆菌-龟分枝杆菌复合群菌株rRNA基因的限制性片段长度多态性（RFLP）。每个DNA样本用EcoRI限制性内切酶切割，片段通过琼脂糖凝胶电泳分离并转移到硝酸纤维素膜上。通过与γ-32P标记的rRNA杂交鉴定含rRNA基因的DNA片段。发现其图谱具有种特异性，且两种菌可相互区分。结果表明，该方法可用于偶然分枝杆菌-龟分枝杆菌复合群的快速基因组特征分析。