耻垢分枝杆菌核糖体RNA基因的研究

Study on rRNA genes in Mycobacterium smegmatis.

作者信息

Suzuki Y, Yamada T

机构信息

Research Institute for Microbiol Diseases, Osaka University.

出版信息

Microbiol Immunol. 1988;32(12):1259-62. doi: 10.1111/j.1348-0421.1988.tb01490.x.

DOI:10.1111/j.1348-0421.1988.tb01490.x

PMID:2467181

Abstract

The number of rRNA genes in Mycobacterium smegmatis was examined by hybridization of BamHI and SalI digests of chromosomal DNA with 3'-end-labeled 5S, 16S, 23S rRNA and tRNA. Each RNA probe gave two hybridization bands. The PstI fragments of 6.6 kilobases were cloned to pBR322. The cloned DNA was characterized by restriction endonuclease mapping, DNA-RNA hybridization, and the R-loop technique.

摘要

通过用3'-末端标记的5S、16S、23S rRNA和tRNA对耻垢分枝杆菌染色体DNA的BamHI和SalI酶切片段进行杂交，检测了耻垢分枝杆菌中rRNA基因的数量。每个RNA探针都给出了两条杂交带。将6.6千碱基的PstI片段克隆到pBR322中。通过限制性内切酶图谱分析、DNA-RNA杂交和R环技术对克隆的DNA进行了表征。