Hirabayashi Y, Fujita S C, Kon K, Ando S
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Japan.
J Biol Chem. 1991 Jun 5;266(16):10268-74.
A monoclonal antibody, 188C1, raised against skin tissue from the back of bullfrogs (Rana catesbeiana) was found to recognize a common antigen in neural and intestinal tissues of chicken (Fujita, S. (1989) in Biological Transduction Mechanisms (Kasai, M., Yoshioka, T., and Suzuki, H., eds) pp. 159-177, Japan Scientific Societies Press, Tokyo Japan). The 188C1 antigen was isolated from chick intestinal tissues and characterized as a novel ganglioside by means of Q-Sepharose and Iatrobeads column chromatography, and chemical, immunochemical, and immunohistochemical analyses. The chemical structure was as follows: Gal beta 1-4 GlcNAc beta 1-3Gal beta 1-3GalNAc beta 1-4Gal beta 1-4Glc beta 1-1'Cer Fuc alpha 1-3 NeuAc alpha 2-3 This represents a novel hybrid structure of type 2 Le(x) epitope and GM1 ganglioside core structure, designated as Le(x)-GM1. Monoclonal antibody 188C1 reacted strongly with Le(x)-GM1 on thin layer chromatography, but its reactivity was greatly reduced when sialic acid was removed from the antigen. This indicated that the internal sialic acid residue might participate in antigenicity of the Le(x) determinant. In addition to 188C1, a more specific antibody reacting with Le(x)-GM1 but not with asialo-Le(x)-GM1 was raised by immunizing a rabbit with Le(x)-GM1. TLC/enzyme immunostaining using this specific antibody showed the presence of Le(x)-GM1 in chick intestinal tissue, but not in chick brain.
一种针对牛蛙（Rana catesbeiana）背部皮肤组织产生的单克隆抗体188C1，被发现可识别鸡的神经和肠道组织中的一种共同抗原（藤田，S.（1989年），载于《生物转导机制》（笠井，M.，吉冈，T.，铃木，H.编），第159 - 177页，日本科学协会出版社，东京，日本）。188C1抗原从鸡的肠道组织中分离出来，并通过Q - 琼脂糖和Iatrobeads柱色谱法以及化学、免疫化学和免疫组织化学分析，被鉴定为一种新型神经节苷脂。其化学结构如下：Galβ1 - 4GlcNAcβ1 - 3Galβ1 - 3GalNAcβ1 - 4Galβ1 - 4Glcβ1 - 1'Cer Fucα1 - 3NeuAcα2 - 3 这代表了2型Le(x)表位和GM1神经节苷脂核心结构的一种新型杂合结构，命名为Le(x) - GM1。单克隆抗体188C1在薄层色谱上与Le(x) - GM1强烈反应，但当从抗原中去除唾液酸时，其反应性大大降低。这表明内部唾液酸残基可能参与Le(x)决定簇的抗原性。除了188C1之外，通过用Le(x) - GM1免疫兔子，产生了一种更特异的抗体，该抗体与Le(x) - GM1反应，但不与脱唾液酸Le(x) - GM1反应。使用这种特异性抗体的薄层色谱/酶免疫染色显示鸡肠道组织中存在Le(x) - GM1，但鸡脑中不存在。
