核糖核酸噬菌体ZIK/1感染后大肠杆菌C3000/L的核糖核酸合成及噬菌体ZIK/1核糖核酸的性质
Ribonucleic acid synthesis by Escherichia coli C3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties of coliphage-ZIK/1 ribonucleic acid.
作者信息
Bishop D H
机构信息
Chemical Biology Unit, Department of Zoology, University of Edinburgh.
出版信息
Biochem J. 1965 Oct;97(1):17-26. doi: 10.1042/bj0970017.
Abstract
- A method is described for the preparation and purification of the RNA from the RNA coliphage ZIK/1. 2. Some of the physical characteristics and infective properties of coliphage-ZIK/1 RNA were examined. 3. A method is also described for examining the type and quantity of RNA synthesized after bacteriophage infection. 4. Ribosome synthesis was decreased 15min. after bacteriophage adsorption, bacteriophage RNA was synthesized from 15min. to 120min. after adsorption and intracellular bacteriophages appeared 40min. after adsorption. Cell lysis commenced 60min. after adsorption, and was half complete 20min. later and 90-95% complete 120min. after adsorption. 5. Cell division continued until 40min. after bacteriophage adsorption. 6. Bacterial ribosomes were conserved during the infective process. 7. Intracellular bacteriophage RNA has sedimentation coefficient 28s but after cell lysis it has sedimentation coefficient 10-5s.
摘要
- 描述了一种从RNA噬菌体ZIK/1制备和纯化RNA的方法。2. 检测了噬菌体ZIK/1 RNA的一些物理特性和感染特性。3. 还描述了一种检测噬菌体感染后合成的RNA类型和数量的方法。4. 噬菌体吸附后15分钟核糖体合成减少，吸附后15分钟到120分钟合成噬菌体RNA，吸附后40分钟出现细胞内噬菌体。吸附后60分钟开始细胞裂解，20分钟后完成一半，吸附后120分钟完成90 - 95%。5. 细胞分裂持续到噬菌体吸附后40分钟。6. 细菌核糖体在感染过程中得以保留。7. 细胞内噬菌体RNA的沉降系数为28s，但细胞裂解后沉降系数为10 - 5s。
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1
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Biochim Biophys Acta. 1962 May 14;55:704-18. doi: 10.1016/0006-3002(62)90848-x.
2
Ribonucleic acids. II. Improved preparation of rat-liver ribonucleic acid.核糖核酸。II. 大鼠肝脏核糖核酸制备方法的改进
Biochim Biophys Acta. 1962 Apr 2;55:545-6. doi: 10.1016/0006-3002(62)90988-5.
3
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