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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1966 Sep;100(3):601-13. doi: 10.1042/bj1000601.

The efficiency of extracting nucleic acids from Escherichia coli after five methods of obtaining cell lysis was determined. 2. The recovery of various nucleic acid species isolated after chromatography on methylated albumin-coated kieselguhr was also examined. 3. Double-stranded coliphage-induced RNA was isolated from infected bacteria and its resistance to ribonuclease digestion under various conditions determined. 4. The involvement of double-stranded RNA during the infection process was demonstrated. 5. The time-course of the syntheses in infected cells of double-stranded RNA, DNA, single-stranded coliphage and 16s ribosomal RNA, transfer RNA and ribosomal 23s RNA was examined. 6. It was demonstrated that the syntheses of DNA, transfer RNA and ribosomal RNA decreased 10-15min. after infection. 7. Synthesis of coliphage RNA commenced 10-15min. after infection and double-stranded RNA was also synthesized from about 10min. after coliphage adsorption.

测定了通过五种获得细胞裂解的方法从大肠杆菌中提取核酸的效率。2. 还检测了在甲基化白蛋白包被的硅藻土上进行色谱分离后分离出的各种核酸种类的回收率。3. 从受感染细菌中分离出双链噬菌体诱导的RNA，并测定其在各种条件下对核糖核酸酶消化的抗性。4. 证明了双链RNA在感染过程中的参与。5. 检测了感染细胞中双链RNA、DNA、单链噬菌体以及16s核糖体RNA、转运RNA和核糖体23s RNA合成的时间进程。6. 证明感染后10 - 15分钟，DNA、转运RNA和核糖体RNA的合成减少。7. 噬菌体RNA的合成在感染后10 - 15分钟开始，双链RNA也在噬菌体吸附后约10分钟开始合成。

Biochem J. 1966 Sep;100(3):601-13. doi: 10.1042/bj1000601.

The efficiency of extracting nucleic acids from Escherichia coli after five methods of obtaining cell lysis was determined. 2. The recovery of various nucleic acid species isolated after chromatography on methylated albumin-coated kieselguhr was also examined. 3. Double-stranded coliphage-induced RNA was isolated from infected bacteria and its resistance to ribonuclease digestion under various conditions determined. 4. The involvement of double-stranded RNA during the infection process was demonstrated. 5. The time-course of the syntheses in infected cells of double-stranded RNA, DNA, single-stranded coliphage and 16s ribosomal RNA, transfer RNA and ribosomal 23s RNA was examined. 6. It was demonstrated that the syntheses of DNA, transfer RNA and ribosomal RNA decreased 10-15min. after infection. 7. Synthesis of coliphage RNA commenced 10-15min. after infection and double-stranded RNA was also synthesized from about 10min. after coliphage adsorption.

测定了通过五种获得细胞裂解的方法从大肠杆菌中提取核酸的效率。2. 还检测了在甲基化白蛋白包被的硅藻土上进行色谱分离后分离出的各种核酸种类的回收率。3. 从受感染细菌中分离出双链噬菌体诱导的RNA，并测定其在各种条件下对核糖核酸酶消化的抗性。4. 证明了双链RNA在感染过程中的参与。5. 检测了感染细胞中双链RNA、DNA、单链噬菌体以及16s核糖体RNA、转运RNA和核糖体23s RNA合成的时间进程。6. 证明感染后10 - 15分钟，DNA、转运RNA和核糖体RNA的合成减少。7. 噬菌体RNA的合成在感染后10 - 15分钟开始，双链RNA也在噬菌体吸附后约10分钟开始合成。