针对大肠杆菌的六种核糖核酸噬菌体碱基比率的测定
DETERMINATION OF BASE RATIOS OF SIX RIBONUCLEIC ACID BACTERIOPHAGES SPECIFIC TO ESCHERICHIA COLI.
作者信息
BISHOP D H, BRADLEY D E
出版信息
Biochem J. 1965 Apr;95(1):82-93. doi: 10.1042/bj0950082.
Abstract
- A method is described for the isolation of single-stranded-RNA coliphages. Two of the six RNA coliphages investigated were new strains. 2. The base ratios of six RNA coliphages were determined by labelling the host bacterium with [(32)P]-phosphate, purification of the radioactive coliphages and separation of 2',3'-ribonucleotides liberated by alkaline hydrolysis of the coliphage RNA. 3. All six of the coliphages were morphologically similar, contained single-stranded RNA, and had sedimentation coefficient 80+/-5s. 4. The six RNA coliphages fell into two distinct groups, both serologically and in terms of their RNA base ratios.
摘要
- 本文描述了一种分离单链RNA大肠杆菌噬菌体的方法。所研究的六种RNA大肠杆菌噬菌体中有两种是新菌株。2. 通过用[（32）P] - 磷酸盐标记宿主细菌、纯化放射性噬菌体以及分离由噬菌体RNA碱性水解释放的2'，3'-核糖核苷酸，测定了六种RNA大肠杆菌噬菌体的碱基比率。3. 所有六种噬菌体在形态上相似，含有单链RNA，沉降系数为80±5s。4. 这六种RNA大肠杆菌噬菌体在血清学和RNA碱基比率方面都分为两个不同的组。
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