兔体内的类固醇代谢。[4-C]孕酮代谢产物的胆汁和尿液排泄。
Steroid metabolism in the rabbit. Biliary and urinary excretion of metabolites of [4-C]progesterone.
作者信息
Taylor W, Scratcherd T
机构信息
Department of Physiology, The Medical School, University of Newcastle upon Tyne.
出版信息
Biochem J. 1965 Oct;97(1):89-94. doi: 10.1042/bj0970089.
Abstract
- [4-(14)C]Progesterone was administered intravenously to anaesthetized male and female New Zealand White rabbits as a single injection or as a 45-60min. infusion. 2. After a single dose about 60% of the radioactivity was recovered in 6hr., and twice as much radioactivity was present in bile as in urine. After infusion total recovery of radioactivity was only about 40% in 6hr., but the relative proportions of metabolites in bile and urine were about the same as after a single dose. 3. Bile and urine samples were hydrolysed successively by beta-glucuronidase, cold acid and hot acid. 4. In bile the major proportion of metabolites appeared in the glucuronide fraction; in urine beta-glucuronidase hydrolysis yielded the greatest amounts of ether-extractable radioactivity, but the greatest proportion of radioactivity could not be extracted by ether from an alkaline solution of the hydrolysed urine. 5. There was no apparent difference in the quantity or distribution of metabolites excreted by male and female animals.
摘要
- 将[4-(14)C]孕酮以单次注射或45 - 60分钟输注的方式静脉注射给麻醉后的雄性和雌性新西兰白兔。2. 单次给药后，约60%的放射性在6小时内被回收，胆汁中的放射性是尿液中的两倍。输注后，6小时内放射性的总回收率仅约为40%，但胆汁和尿液中代谢物的相对比例与单次给药后大致相同。3. 胆汁和尿液样本依次用β-葡萄糖醛酸酶、冷酸和热酸水解。4. 在胆汁中，代谢物的主要部分出现在葡萄糖醛酸苷部分；在尿液中，β-葡萄糖醛酸酶水解产生的可乙醚萃取的放射性量最大，但从水解尿液的碱性溶液中，最大比例的放射性不能被乙醚萃取。5. 雄性和雌性动物排泄的代谢物的数量和分布没有明显差异。
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