Zhao Lin-qing, Qian Yuan, Zhu Ru-nan, Deng Jie, Wang Fang
Laboratory of Virology, Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Feb;27(2):154-6.
To develop a rapid, sensitive and specific method for detection human rhinovirus (HRV) from clinical specimens.
Primers derived from the highly conserved 5'noncoding region of human rhinovirus were used to develop a nested RT-PCR for detecting HRV. The sensitivity and specificity of the RT-PCR were determined using various RNA while DNA viruses were used as control. Seven hundred and seventy-one specimens collected from children with symptoms of acute respiratory infections from Nov. 2002 to Oct. 2003 were analyzed for HRV by RT-PCR as well as for other respiratory viruses through isolation of virus and indirect immunofluorescent assay.
Only the cDNA from HRV was positive by RT-PCR, indicating the nested RT-PCR was specific. With RT-PCR, HRV were detected in 148 out of 771 specimens (19.2%). As for HRV positive rates, it was found 53.3% in pharyngitis patients; 43.8% in laryngitis patients and 28.7% in bronchitis patients. In Sep. 2002 and from Aug. 2003 to Oct. 2003, HRV positive rates were high (21.6% - 32.6%), with Sep. 2003 in particular--32.6%. From Mar. 2003 to Jul. 2003, HRV positive rates maintained from 16.0% to 19.1%.
HRV was one of the important agents for acute respiratory infections in infants and young children in Beijing.
建立一种从临床标本中快速、灵敏且特异检测人鼻病毒（HRV）的方法。
使用源自人鼻病毒高度保守的5'非编码区的引物，开发用于检测HRV的巢式逆转录聚合酶链反应（RT-PCR）。利用各种RNA测定RT-PCR的敏感性和特异性，同时以DNA病毒作为对照。对2002年11月至2003年10月期间从有急性呼吸道感染症状的儿童中收集的771份标本，通过RT-PCR分析HRV，并通过病毒分离和间接免疫荧光测定法分析其他呼吸道病毒。
RT-PCR仅显示来自HRV的互补DNA（cDNA）呈阳性，表明巢式RT-PCR具有特异性。通过RT-PCR，在771份标本中的148份（19.2%）检测到HRV。至于HRV阳性率，在咽炎患者中为53.3%；在喉炎患者中为43.8%，在支气管炎患者中为28.7%。2002年9月以及2003年8月至10月期间，HRV阳性率较高（21.6% - 32.6%），尤其是2003年9月——为32.6%。2003年3月至7月期间，HRV阳性率维持在16.0%至19.1%之间。
HRV是北京婴幼儿急性呼吸道感染的重要病原体之一。
