评估一种用于从钢表面回收炭疽芽孢杆菌孢子的大泡沫拭子方案。
Evaluation of a macrofoam swab protocol for the recovery of Bacillus anthracis spores from a steel surface.
作者信息
Hodges L R, Rose L J, Peterson A, Noble-Wang J, Arduino M J
机构信息
Centers for Disease Control and Prevention, 1600 Clifton Rd., Mail Stop C16, Atlanta, GA 30333, USA.
出版信息
Appl Environ Microbiol. 2006 Jun;72(6):4429-30. doi: 10.1128/AEM.02923-05.
Abstract
A protocol to recover Bacillus anthracis spores from a steel surface using macrofoam swabs was evaluated for its accuracy, precision, reproducibility, and limit of detection. Macrofoam swabs recovered 31.7 to 49.1% of spores from 10-cm2 steel surfaces with a < or =32.7% coefficient of variation in sampling precision and reproducibility for inocula of > or =38 spores.
摘要
对一种使用大泡沫拭子从钢表面回收炭疽芽孢杆菌孢子的方案进行了准确性、精密度、重现性和检测限评估。对于接种量≥38个孢子的情况，大泡沫拭子从10平方厘米钢表面回收了31.7%至49.1%的孢子，采样精密度和重现性的变异系数≤32.7%。
相似文献
1
Evaluation of a macrofoam swab protocol for the recovery of Bacillus anthracis spores from a steel surface.
Appl Environ Microbiol. 2006 Jun;72(6):4429-30. doi: 10.1128/AEM.02923-05.
2
National validation study of a swab protocol for the recovery of Bacillus anthracis spores from surfaces.
J Microbiol Methods. 2010 May;81(2):141-6. doi: 10.1016/j.mimet.2010.02.010. Epub 2010 Mar 1.
3
Most-probable-number rapid viability PCR method to detect viable spores of Bacillus anthracis in swab samples.
J Microbiol Methods. 2010 May;81(2):200-2. doi: 10.1016/j.mimet.2010.02.011. Epub 2010 Mar 1.
4
False-negative rate, limit of detection and recovery efficiency performance of a validated macrofoam-swab sampling method for low surface concentrations of Bacillus anthracis Sterne and Bacillus atrophaeus spores.
J Appl Microbiol. 2016 Jul;121(1):149-62. doi: 10.1111/jam.13128. Epub 2016 May 6.
5
Comparison of false-negative rates and limits of detection following macrofoam-swab sampling of Bacillus anthracis surrogates via Rapid Viability PCR and plate culture.
J Appl Microbiol. 2018 May;124(5):1092-1106. doi: 10.1111/jam.13706. Epub 2018 Mar 13.
6
Swab materials and Bacillus anthracis spore recovery from nonporous surfaces.
Emerg Infect Dis. 2004 Jun;10(6):1023-9. doi: 10.3201/eid1006.030716.
7
Unsupported conclusions on the Bacillus anthracis spores.
Appl Environ Microbiol. 2007 Aug;73(15):5074. doi: 10.1128/AEM.02898-06.
8
Evaluation of Bacillus anthracis and Yersinia pestis sample collection from nonporous surfaces by quantitative real-time PCR.
Lett Appl Microbiol. 2010 Apr;50(4):431-7. doi: 10.1111/j.1472-765X.2010.02821.x. Epub 2010 Feb 8.
9
National validation study of a cellulose sponge wipe-processing method for use after sampling Bacillus anthracis spores from surfaces.
Appl Environ Microbiol. 2011 Dec;77(23):8355-9. doi: 10.1128/AEM.05377-11. Epub 2011 Sep 30.
10
Use of onsite technologies for rapidly assessing environmental Bacillus anthracis contamination on surfaces in buildings.
MMWR Morb Mortal Wkly Rep. 2001 Dec 7;50(48):1087.
引用本文的文献
1
Evaluation of sponge wipe surface sampling for collection of potential surrogates for non-spore-forming bioterrorism agents.
J Appl Microbiol. 2024 May 1;135(5). doi: 10.1093/jambio/lxae097.
2
Microbiome and Metagenome Analyses of a Closed Habitat during Human Occupation.
mSystems. 2020 Jul 28;5(4):e00367-20. doi: 10.1128/mSystems.00367-20.
3
Development and Comparison of Complementary Methods to Study Potential Skin and Inhalational Exposure to Pathogens During Personal Protective Equipment Doffing.
Clin Infect Dis. 2019 Sep 13;69(Suppl 3):S231-S240. doi: 10.1093/cid/ciz616.
4
Microbiomes of China's Space Station During Assembly, Integration, and Test Operations.
Microb Ecol. 2019 Oct;78(3):631-650. doi: 10.1007/s00248-019-01344-4. Epub 2019 Feb 26.
5
Alternative fast analysis method for cellulose sponge surface sampling wipes with low concentrations of Bacillus Spores.
J Microbiol Methods. 2019 Jan;156:5-8. doi: 10.1016/j.mimet.2018.11.013. Epub 2018 Nov 16.
6
Efficacy of a Sonicating Swab for Removal and Capture of Microorganisms from Experimental and Natural Contaminated Surfaces.
Appl Environ Microbiol. 2018 Apr 16;84(9). doi: 10.1128/AEM.00208-18. Print 2018 May 1.
7
Efficacy of a Sonicating Swab for Removal and Capture of Listeria monocytogenes in Biofilms on Stainless Steel.
Appl Environ Microbiol. 2017 May 17;83(11). doi: 10.1128/AEM.00109-17. Print 2017 Jun 1.
8
Evaluating Composite Sampling Methods of Bacillus Spores at Low Concentrations.
PLoS One. 2016 Oct 13;11(10):e0164582. doi: 10.1371/journal.pone.0164582. eCollection 2016.
9
Storage Effects on Sample Integrity of Environmental Surface Sampling Specimens with Spores.
Biosafety (Los Angel). 2013;2013(Suppl 1):002. doi: 10.4172/2167-0331.S1-002. Epub 2013 Jan 17.
10
Effect of Surface Sampling and Recovery of Viruses and Non-Spore-Forming Bacteria on a Quantitative Microbial Risk Assessment Model for Fomites.
Environ Sci Technol. 2016 Jun 7;50(11):5945-52. doi: 10.1021/acs.est.5b06275. Epub 2016 May 18.
本文引用的文献
1
Evaluation of the Biological Sampling Kit (BiSKit) for large-area surface sampling.
Appl Environ Microbiol. 2004 Dec;70(12):7040-5. doi: 10.1128/AEM.70.12.7040-7045.2004.
2
Swab materials and Bacillus anthracis spore recovery from nonporous surfaces.
Emerg Infect Dis. 2004 Jun;10(6):1023-9. doi: 10.3201/eid1006.030716.
3
Bacillus anthracis contamination and inhalational anthrax in a mail processing and distribution center.
J Appl Microbiol. 2004;96(5):1048-56. doi: 10.1111/j.1365-2672.2004.02223.x.
4
Responding to a bioterrorist attack: environmental investigation of anthrax in New Jersey.
Appl Occup Environ Hyg. 2003 Oct;18(10):780-5. doi: 10.1080/10473220301445.
5
A field investigation of Bacillus anthracis contamination of U.S. Department of Agriculture and other Washington, D.C., buildings during the anthrax attack of October 2001.
Appl Environ Microbiol. 2003 Jan;69(1):593-9. doi: 10.1128/AEM.69.1.593-599.2003.
6
Surface sampling methods for Bacillus anthracis spore contamination.
Emerg Infect Dis. 2002 Oct;8(10):1145-51. doi: 10.3201/eid0810.020382.
7
Environmental sampling for spores of Bacillus anthracis.
Emerg Infect Dis. 2002 Oct;8(10):1083-7. doi: 10.3201/eid0810.020398.
8
Inhalational anthrax outbreak among postal workers, Washington, D.C., 2001.
Emerg Infect Dis. 2002 Oct;8(10):1066-72. doi: 10.3201/eid0810.020330.
9
Microbiologic assay of space hardware.
Environ Biol Med. 1971 May;1(1):27-36.
10
Microbiological sampling of surfaces.
J Appl Bacteriol. 1968 Sep;31(3):336-43. doi: 10.1111/j.1365-2672.1968.tb00375.x.
Zotero 插件