Jacobs Technology, Inc., 600 William Northern Blvd, Tullahoma, TN 37388, United States.
Office of Research and Development, US EPA, Research Triangle Park, NC 27711, United States.
J Appl Microbiol. 2024 May 1;135(5). doi: 10.1093/jambio/lxae097.
Evaluate the efficacy of sponge wipe sampling at recovering potential bacterial surrogates for Category A and B non-spore-forming bacterial bioterrorism agents from hard, nonporous surfaces.
A literature survey identified seven nonpathogenic bacteria as potential surrogates for selected Category A and B non-spore-forming bacterial agents. Small (2 × 4 cm) and large (35.6 × 35.6 cm) coupons made from either stainless steel, plastic, or glass, were inoculated and utilized to assess persistence and surface sampling efficiency, respectively. Three commercially available premoistened sponge wipes (3M™, Sani-Stick®, and Solar-Cult®) were evaluated.
Mean recoveries from persistence testing indicated that three microorganisms (Yersinia ruckeri, Escherichia coli, and Serratia marcescens) demonstrated sufficient persistence across all tested material types. Sampling of large inoculated (≥107 CFU per sample) coupons resulted in mean recoveries ranging from 6.6 to 3.4 Log10 CFU per sample. Mean recoveries for the Solar-Cult®, 3M™ sponge wipes, and Sani-Sticks® across all test organisms and all material types were ≥5.7, ≥3.7, and ≥3.4 Log10 CFU per sample, respectively. Mean recoveries for glass, stainless steel, and ABS plastic across all test organisms and all sponge types were ≥3.8, ≥3.7, and ≥3.4 Log10 CFU per sample, respectively.
Recovery results suggest that sponge wipe sampling can effectively be used to recover non-spore-forming bacterial cells from hard, nonporous surfaces such as stainless steel, ABS plastic, and glass.
评估海绵擦拭取样法从硬的、非多孔表面回收 A 类和 B 类非孢子形成细菌生物恐怖剂潜在细菌替代品的效果。
文献综述确定了七种非致病性细菌作为选定的 A 类和 B 类非孢子形成细菌制剂的潜在替代品。从小（2×4 厘米）到大（35.6×35.6 厘米）的不锈钢、塑料或玻璃制成的小（2×4 厘米）和大（35.6×35.6 厘米）小方块进行接种，并分别用于评估持久性和表面取样效率。评估了三种市售的预湿海绵擦拭器（3M™，Sani-Stick®和 Solar-Cult®）。
持久性测试的平均回收率表明，三种微生物（鲁氏耶尔森氏菌、大肠杆菌和粘质沙雷氏菌）在所有测试的材料类型中均表现出足够的持久性。对大接种量（每个样品≥107 CFU）的小方块进行采样，平均回收率范围为每个样品 6.6 至 3.4 Log10 CFU。所有测试生物和所有材料类型的 Solar-Cult®、3M™海绵擦拭器和 Sani-Sticks®的平均回收率分别为≥5.7、≥3.7 和≥3.4 Log10 CFU 每个样品。所有测试生物和所有海绵类型的玻璃、不锈钢和 ABS 塑料的平均回收率分别为≥3.8、≥3.7 和≥3.4 Log10 CFU 每个样品。
回收结果表明，海绵擦拭取样法可有效地从硬的、非多孔表面（如不锈钢、ABS 塑料和玻璃）回收非孢子形成细菌细胞。
