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通过体内重组进行的缺失实验表明，来自苏云金芽孢杆菌以色列亚种的28千道尔顿溶细胞多肽对杀蚊活性并非必不可少。

Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity.

作者信息

Delécluse A, Charles J F, Klier A, Rapoport G

机构信息

Unité de Biochimie Microbienne, URA 1300 CNRS, Paris, France.

出版信息

J Bacteriol. 1991 Jun;173(11):3374-81. doi: 10.1128/jb.173.11.3374-3381.1991.

DOI:10.1128/jb.173.11.3374-3381.1991
PMID:1675212
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207948/
Abstract

The cytA gene encoding the 28-kDa polypeptide of Bacillus thuringiensis subsp. israelensis crystals was disrupted in the 72-MDa resident plasmid by in vivo recombination, thus indicating that homologous recombination occurs in B. thuringiensis. The absence of the 28-kDa protein in B. thuringiensis did not affect the crystallization of the other toxic components of the parasporal body (68-, 125-, and 135-kDa polypeptides). The absence of the 28-kDa protein abolished the hemolytic activity of B. thuringiensis subsp. israelensis crystals. However, the mosquitocidal activity of the 28-kDa protein-free crystals did not differ significantly from that of the wild-type crystals when tested on Aedes aegypti and Culex pipiens larvae. The 28-kDa protein contributed slightly to the toxicity to Anopheles stephensi larvae. This indicates that the 28-kDa protein is not essential for mosquitocidal activity, at least against the three species tested.

摘要

编码苏云金芽孢杆菌以色列亚种晶体中28 kDa多肽的cytA基因通过体内重组在72 kDa的常驻质粒中被破坏，这表明同源重组发生在苏云金芽孢杆菌中。苏云金芽孢杆菌中不存在28 kDa蛋白并不影响伴孢晶体其他毒性成分（68 kDa、125 kDa和135 kDa多肽）的结晶。28 kDa蛋白的缺失消除了苏云金芽孢杆菌以色列亚种晶体的溶血活性。然而，在对埃及伊蚊和致倦库蚊幼虫进行测试时，不含28 kDa蛋白的晶体的杀蚊活性与野生型晶体的杀蚊活性没有显著差异。28 kDa蛋白对斯氏按蚊幼虫的毒性贡献较小。这表明28 kDa蛋白对于杀蚊活性不是必需的，至少对所测试的三个物种而言如此。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c555/207948/9cd5f4bc363f/jbacter00101-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c555/207948/d67487768b4b/jbacter00101-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c555/207948/9f88e12f3c3b/jbacter00101-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c555/207948/9cd5f4bc363f/jbacter00101-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c555/207948/d67487768b4b/jbacter00101-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c555/207948/9f88e12f3c3b/jbacter00101-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c555/207948/9cd5f4bc363f/jbacter00101-0117-a.jpg

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