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CryIVD多肽在苏云金芽孢杆菌以色列亚种整体毒性中的作用。

Role of the CryIVD polypeptide in the overall toxicity of Bacillus thuringiensis subsp. israelensis.

作者信息

Poncet S, Anello G, Delécluse A, Klier A, Rapoport G

机构信息

Unité de Biochimie Microbienne, Institut Pasteur, URA 1300 du Centre National de la Recherche Scientific, Paris, France.

出版信息

Appl Environ Microbiol. 1993 Nov;59(11):3928-30. doi: 10.1128/aem.59.11.3928-3930.1993.

DOI:10.1128/aem.59.11.3928-3930.1993
PMID:8285695
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC182551/
Abstract

The gene encoding the CryIVD protein of B. thuringiensis subsp. israelensis crystals was disrupted by in vivo recombination. The toxicity of the CryIVD protein-free inclusions was similar to that of the wild-type crystals on Anopheles stephensi larvae but was half the wild-type toxicity on Culex pipiens and Aedes aegypti larvae.

摘要

苏云金芽孢杆菌以色列亚种晶体的CryIVD蛋白编码基因通过体内重组被破坏。不含CryIVD蛋白的包涵体对斯氏按蚊幼虫的毒性与野生型晶体相似，但对致倦库蚊和埃及伊蚊幼虫的毒性仅为野生型的一半。

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Role of the CryIVD polypeptide in the overall toxicity of Bacillus thuringiensis subsp. israelensis.CryIVD多肽在苏云金芽孢杆菌以色列亚种整体毒性中的作用。
Appl Environ Microbiol. 1993 Nov;59(11):3928-30. doi: 10.1128/aem.59.11.3928-3930.1993.
2
Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity.通过体内重组进行的缺失实验表明，来自苏云金芽孢杆菌以色列亚种的28千道尔顿溶细胞多肽对杀蚊活性并非必不可少。
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本文引用的文献

1
Expression of cryIVA and cryIVB Genes, Independently or in Combination, in a Crystal-Negative Strain of Bacillus thuringiensis subsp. israelensis.表达 cryIVA 和 cryIVB 基因，独立或组合，在一个晶体阴性菌株的苏云金芽孢杆菌亚种。以色列。
Appl Environ Microbiol. 1993 Nov;59(11):3922-7. doi: 10.1128/aem.59.11.3922-3927.1993.
2
High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes.苏云金芽孢杆菌中高水平的cryIVD和cytA基因表达不需要20千道尔顿蛋白，并且共表达的基因产物对蚊子的毒性具有协同作用。
Appl Environ Microbiol. 1993 Mar;59(3):815-21. doi: 10.1128/aem.59.3.815-821.1993.
3
苏云金芽孢杆菌以色列亚种的一种编码毒素的大质粒向球形芽孢杆菌杀蚊菌株的接合转移
Appl Environ Microbiol. 2006 Mar;72(3):1766-70. doi: 10.1128/AEM.72.3.1766-1770.2006.
4
Bacillus thuringiensis and its pesticidal crystal proteins.苏云金芽孢杆菌及其杀虫晶体蛋白。
Microbiol Mol Biol Rev. 1998 Sep;62(3):775-806. doi: 10.1128/MMBR.62.3.775-806.1998.
5
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Appl Environ Microbiol. 1997 Nov;63(11):4413-20. doi: 10.1128/aem.63.11.4413-4420.1997.
6
Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.来自苏云金芽孢杆菌jegathesan亚种的一个编码高杀蚊蛋白的新型毒素基因的克隆与表达。
Appl Environ Microbiol. 1995 Dec;61(12):4230-5. doi: 10.1128/aem.61.12.4230-4235.1995.
7
Transcriptional regulation of the cryIVD gene operon from Bacillus thuringiensis subsp. israelensis.苏云金芽孢杆菌以色列亚种cryIVD基因操纵子的转录调控
J Bacteriol. 1995 May;177(9):2283-91. doi: 10.1128/jb.177.9.2283-2291.1995.
Correlation between specific plasmids and delta-endotoxin production in Bacillus thuringiensis.
苏云金芽孢杆菌中特定质粒与δ-内毒素产生之间的相关性。
Plasmid. 1981 May;5(3):352-65. doi: 10.1016/0147-619x(81)90010-x.
4
Generalized transduction in Bacillus thuringiensis var. berliner 1715 using bacteriophage CP-54Ber.使用噬菌体CP-54Ber对苏云金芽孢杆菌柏林变种1715进行广义转导
J Gen Microbiol. 1980 Nov;121(1):203-12. doi: 10.1099/00221287-121-1-203.
5
Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.pC194质粒的核苷酸序列及功能图谱，该质粒可产生诱导型氯霉素抗性。
J Bacteriol. 1982 May;150(2):815-25. doi: 10.1128/jb.150.2.815-825.1982.
6
Bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo.苏云金芽孢杆菌以色列变种晶体δ-内毒素：对昆虫和哺乳动物细胞的体外及体内作用
J Cell Sci. 1983 Mar;60:181-97. doi: 10.1242/jcs.60.1.181.
7
Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. "kurstaki" and "israelensis".苏云金芽孢杆菌亚种“库尔斯塔克”和“以色列亚种”中鳞翅目和双翅目特异性杀虫内毒素基因之间的结构相似性。
J Bacteriol. 1986 Jun;166(3):801-11. doi: 10.1128/jb.166.3.801-811.1986.
8
Specificity of action on mosquito larvae of Bacillus thuringiensis israelensis toxins encoded by two different genes.由两个不同基因编码的苏云金芽孢杆菌以色列亚种毒素对蚊虫幼虫作用的特异性
Mol Gen Genet. 1988 Sep;214(1):42-7. doi: 10.1007/BF00340177.
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Molecular characterization of a gene encoding a 72-kilodalton mosquito-toxic crystal protein from Bacillus thuringiensis subsp. israelensis.来自苏云金芽孢杆菌以色列亚种的编码一种72千道尔顿蚊虫毒性晶体蛋白的基因的分子特征分析。
J Bacteriol. 1988 Oct;170(10):4732-8. doi: 10.1128/jb.170.10.4732-4738.1988.
10
Insecticidal crystal proteins of Bacillus thuringiensis.苏云金芽孢杆菌的杀虫晶体蛋白
Microbiol Rev. 1989 Jun;53(2):242-55. doi: 10.1128/mr.53.2.242-255.1989.