Caruso B, Senna G, Rizzotti P
Laboratory of Clinical Chemistry, Ospedale Civile Maggiore Borgo Trento, Verona.
Eur Ann Allergy Clin Immunol. 2006 Mar;38(3):77-82.
At present test tube allergy diagnosis is becoming increasingly more comparable to skin prick tests and is therefore increasingly more reliable, not only from an analytical point of view but also from a clinical one. The cost of test tube allergens has decreased over the years and the specific IgE dosage can quickly give a good diagnostic indication.
To study the percentage of positive subjects for each individual allergen in cases of suspected allergy, the laboratory can easily identify, also by age bracket, the positivity for those well known allergens that are more commonly responsible for allergic pathologies by using the skin prick test. Our laboratory has studied the test tube diagnostic activity of 2002 and 2003. The inhaled allergens used to identify the positivity percentage were selected from those in our Allergy Unit and which literature identifies as those more commonly positive at skin prick tests.
The positivity rate of specific IgEs (UniCAP100- Phamarcia) were analyses for two age brackets before and after the age of 12. The younger than 12 group was then subdivided further into pre-school age (3-5 years) and school age (6-12).
It can be stated that in the grass group, the seasonal allergens, the most commonly positive were cereals and pellitory, the latter increasing in adult age (above 12 years). In the tree group of seasonal allergens, positivity was found to increase in adult age for olive and cypress trees. The more commonly positive perennial allergens in the adult age (above 12) are dermathophagoides and cat. Below 12, there is a strong positivity to alternaria.
Such test tube studies on IgE positivity are not only useful for better defining diagnostic patterns to give an initial idea of suspected allergy, but also to highlight any changes in the IgE antibody count within different age brackets, with the possibility of documenting the progress of the pathology which is characteristic of the allergy in question.
目前，试管过敏诊断与皮肤点刺试验的可比性越来越高，因此不仅从分析角度，而且从临床角度来看都越来越可靠。多年来，试管过敏原的成本有所下降，特异性IgE检测能够快速提供良好的诊断依据。
研究疑似过敏病例中每种过敏原的阳性受试者百分比，实验室可以通过皮肤点刺试验，轻松地按年龄组确定那些更常见的引起过敏病变的知名过敏原的阳性情况。我们实验室研究了2002年和2003年的试管诊断活动。用于确定阳性百分比的吸入性过敏原是从我们过敏科的过敏原中挑选出来的，并且文献中也将其确定为在皮肤点刺试验中更常见的阳性过敏原。
分析了12岁前后两个年龄组特异性IgE（UniCAP100 - 法玛西亚）的阳性率。12岁以下的组进一步细分为学龄前（3 - 5岁）和学龄期（6 - 12岁）。
可以说，在草类组（季节性过敏原）中，最常见的阳性过敏原是谷类和墙草，后者在成年期（12岁以上）有所增加。在树木类季节性过敏原组中，橄榄树和柏树的阳性率在成年期有所增加。成年期（12岁以上）更常见的常年性阳性过敏原是尘螨和猫。12岁以下，链格孢属的阳性率很高。
这种关于IgE阳性的试管研究不仅有助于更好地确定诊断模式，以便初步了解疑似过敏情况，还能突出不同年龄组内IgE抗体计数的任何变化，有可能记录所讨论过敏的病理学进展特征。
