全香烟烟雾暴露与香烟烟雾提取物对小鼠纤毛气管上皮的体内差异效应。
Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium.
作者信息
Elliott Margaret K, Sisson Joseph H, West William W, Wyatt Todd A
机构信息
Pulmonary, Critical Care and Sleep Medicine Section, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA.
出版信息
Exp Lung Res. 2006 Mar-Apr;32(3-4):99-118. doi: 10.1080/01902140600710546.
Abstract
In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.
摘要
在本研究中，作者比较了气相香烟烟雾（CS）与香烟烟雾提取物（CSE）对C57BL/6小鼠肺部和上呼吸道的影响。作者发现，与CS和对照组相比，CSE处理显著增加了中性粒细胞流入（P <.001）、基线纤毛摆动频率（CBF）（P <.05）和蛋白激酶C活性。异丙肾上腺素在CS暴露时增加CBF，但在CSE暴露时降低CBF（P <.01）。异丙肾上腺素在除CSE外的所有组中增加蛋白激酶A（PKA）活性。CSE暴露诱发炎症性细胞细支气管炎。这些数据表明，与CS暴露相比，CSE暴露对肺部和气管上皮有不同的影响。
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