Lu Peng, Li Yun, Gorman Amanda, Chi Young-In
Department of Molecular and Cellular Biochemistry and Center for Structural Biology, University of Kentucky, Lexington, KY 40536, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt 6):525-9. doi: 10.1107/S1744309106015168. Epub 2006 May 5.
Hepatocyte nuclear factor 1beta (HNF1beta) is a member of the POU transcription-factor family and binds the target DNA as a dimer with nanomolar affinity. The HNF1beta-DNA complex has been prepared and crystallized by hanging-drop vapor diffusion in 6%(v/v) PEG 300, 5%(w/v) PEG 8000, 8%(v/v) glycerol and 0.1 M Tris pH 8.0. The crystals diffracted to 3.2 A (93.9% completeness) using a synchrotron-radiation source under cryogenic (100 K) conditions and belong to space group R3, with unit-cell parameters a = b = 172.69, c = 72.43 A. A molecular-replacement solution has been obtained and structure refinement is in progress. This structure will shed light on the molecular mechanism of promoter recognition by HNF1beta and the molecular basis of the disease-causing mutations found in it.
肝细胞核因子1β（HNF1β）是POU转录因子家族的成员，以纳摩尔亲和力作为二聚体结合靶DNA。HNF1β-DNA复合物已通过悬滴气相扩散法在6%（v/v）聚乙二醇300、5%（w/v）聚乙二醇8000、8%（v/v）甘油和0.1M Tris pH 8.0中制备并结晶。使用同步辐射源在低温（100K）条件下，晶体衍射至3.2 Å（完整性为93.9%），属于R3空间群，晶胞参数a = b = 172.69，c = 72.43 Å。已获得分子置换解，结构精修正在进行中。该结构将阐明HNF1β识别启动子的分子机制以及其中发现的致病突变的分子基础。
