Haber J, Grinnell C M, Beem J E, Clark W B
Department of Oral Pathology, School of Dental Medicine, Tufts University, Boston, Massachusetts 02111.
Infect Immun. 1991 Jul;59(7):2364-9. doi: 10.1128/iai.59.7.2364-2369.1991.
Antibodies reactive with type 1 and type 2 fimbriae from Actinomyces viscosus T14V specifically inhibit the adherence of A. viscosus T14V to salivary pellicle-coated tooth surfaces and other bacteria, and these antibodies are thought to modulate colonization by this microorganism. These studies were done to determine whether previously noted differences in the antibody responses of inbred mice to type 1 and type 2 fimbriae might be under genetic control. The serum immunoglobulin G (IgG) and IgM antibody responses of inbred, F1 hybrid, and H-2 congenic mice, immunized with A. viscosus T14V cells, were analyzed by enzyme-linked immunosorbent assays for antibodies reactive with A. viscosus T14V whole-cell type 1 and type 2 fimbriae. The results confirmed earlier findings and indicated striking variations in the amounts of IgG anti-type 1 (23-fold) and anti-type 2 (48-fold) fimbria antibodies elicited. The responses of the 17 inbred strains tested showed a relatively continuous distribution from high to low, as well as marked differences in the responses of H-2 and Igh-C identical strain pairs. An analysis of the responses of F1 hybrid and H-2 congenic mice indicated dominance of the low-responder gene(s) and control by H-2-linked genes. Antisera from two high-responder strains inhibited in vitro bacterial adherence to a much greater degree than antisera from a low-responding strain. These data suggest polygenic control of the magnitude of the IgG anti-type 1 and anti-type 2 fimbria antibody responses by H-2-linked genes as well as background genes not associated with H-2 or Igh-C loci.
与粘性放线菌T14V 1型和2型菌毛发生反应的抗体能特异性抑制粘性放线菌T14V对唾液薄膜包被的牙齿表面及其他细菌的黏附，并且这些抗体被认为可调节该微生物的定植。开展这些研究是为了确定之前所发现的近交系小鼠对1型和2型菌毛抗体反应的差异是否可能受基因控制。通过酶联免疫吸附测定法分析了用粘性放线菌T14V细胞免疫的近交系、F1杂种和H-2同源小鼠血清中的免疫球蛋白G（IgG）和IgM抗体反应，以检测与粘性放线菌T14V全细胞1型和2型菌毛发生反应的抗体。结果证实了早期研究结果，并表明所诱导产生的IgG抗1型（23倍）和抗2型（48倍）菌毛抗体量存在显著差异。所测试的17个近交系的反应显示出从高到低的相对连续分布，以及H-2和Igh-C相同品系对反应的显著差异。对F1杂种和H-2同源小鼠反应的分析表明低反应基因具有显性，且受H-2连锁基因控制。来自两个高反应品系的抗血清在体外对细菌黏附的抑制程度远高于来自低反应品系的抗血清。这些数据表明，IgG抗1型和抗2型菌毛抗体反应的强度受H-2连锁基因以及与H-2或Igh-C基因座无关的背景基因的多基因控制。
