Kimura S, Tada N, Nakayama E, Liu Y, Hämmerling U
Immunogenetics. 1981;14(1-2):3-14. doi: 10.1007/BF00344295.
Five monoclonal antibodies were established by the fusion of mouse myeloma cells (NS.1) with spleen cells from A and (A x C3H/An)F1 mice hyperimmunized with 70Z/3 tumor cells. These antibodies recognized a new antigenic specificity provisionally called Ly-m20.2. In direct cytotoxicity assays, 60 percent of cells in spleen, 40 percent in lymph node, 50 percent in bone marrow and less than 5 percent in thymus were found to react with three of the five antibodies, whereas the two others yielded somewhat lower cytotoxicity indices. The Ly-m20.2 antigen was also expressed on cells derived from liver and kidney but not on cells derived from brain. As judged from cytotoxicity assays with separated T and B cells, Ly-m20.2 antigen is carried preferentially on B lymphocytes. Direct plaque-forming cells (PFC) were completely eliminated by Ly-m20.2-specific antibody and complement. Linkage tests by analysis in 20 (CBA/J x C3H/An) x C3H/An backcross mice and by segregation analysis of BXH and SWXL recombinant inbred strains indicate close association of the loci controlling Ly-m20.2 and M1s antigens on chromosome 1.
通过将小鼠骨髓瘤细胞（NS.1）与用70Z/3肿瘤细胞超免疫的A和（A×C3H/An）F1小鼠的脾细胞融合，建立了五种单克隆抗体。这些抗体识别一种新的抗原特异性，暂称为Ly-m20.2。在直接细胞毒性试验中，发现五种抗体中的三种能与脾中60%的细胞、淋巴结中40%的细胞、骨髓中50%的细胞发生反应，而胸腺中不到5%的细胞发生反应，另外两种抗体产生的细胞毒性指数略低。Ly-m20.2抗原也在源自肝脏和肾脏的细胞上表达，但不在源自大脑的细胞上表达。从对分离的T细胞和B细胞进行的细胞毒性试验判断，Ly-m20.2抗原优先存在于B淋巴细胞上。直接空斑形成细胞（PFC）被Ly-m20.2特异性抗体和补体完全消除。通过对20只（CBA/J×C3H/An）×C3H/An回交小鼠进行分析以及对BXH和SWXL重组近交系进行分离分析的连锁试验表明，控制Ly-m20.2和M1s抗原的基因座在1号染色体上紧密连锁。
