Kelly E A, Cruz E S, Hauda K M, Wassom D L
Department of Pathobiological Sciences, School of Veterinary Medicine, University Wisconsin-Madison 53706.
J Immunol. 1991 Jul 1;147(1):306-11.
The differential induction of cytokines associated with Th1 and Th2 subsets has recently been described during Trichinella spiralis infection. Increased levels of resistance appear to correlate with elevated levels of the Th1-associated cytokines, IFN-gamma and IL-2. In the present report, a filter immunoplaque assay is used to quantify the actual numbers of cells that secrete IFN-gamma and IL-5. It is demonstrated that, in T. spiralis-infected B10.Q mice, Th1- and Th2-associated responses are compartmentalized to different lymphoid organs. Thus, Ag-induced IFN-gamma-producing cells predominate in the spleen, whereas IL-5-producing cells prevail in the mesenteric lymph nodes (MLN). A corresponding compartmentalization of Ag-specific IgA and IgG1 antibody-secreting cells to the MLN is also noted. The virtual absence of Th1-associated responses in the MLN appears to be an Ag-associated phenomenon. MLN from either naive or T. spiralis-infected mice do have the capacity to secrete IFN-gamma if stimulated with Con A. The striking compartmentalization of Ag-driven cytokine responses seen in this parasite system may facilitate study of the mechanisms that regulate the induction of Th1 and Th2 subsets.
最近已描述了旋毛虫感染期间与Th1和Th2亚群相关的细胞因子的差异诱导情况。抵抗力水平的升高似乎与Th1相关细胞因子IFN-γ和IL-2水平的升高相关。在本报告中，采用滤膜免疫菌斑测定法来定量分泌IFN-γ和IL-5的细胞的实际数量。结果表明，在感染旋毛虫的B10.Q小鼠中，Th1和Th2相关反应定位于不同的淋巴器官。因此，抗原诱导的产生IFN-γ的细胞在脾脏中占主导，而产生IL-5的细胞在肠系膜淋巴结（MLN）中占优势。还注意到抗原特异性IgA和IgG1抗体分泌细胞相应地定位于MLN。MLN中几乎不存在Th1相关反应似乎是一种与抗原相关的现象。如果用刀豆蛋白A刺激，来自未感染或感染旋毛虫小鼠的MLN确实有分泌IFN-γ的能力。在这个寄生虫系统中观察到的抗原驱动的细胞因子反应的显著定位可能有助于研究调节Th1和Th2亚群诱导的机制。
