Else K J, Grencis R K
Department of Cell & Structural Biology, University of Manchester, U.K.
Immunology. 1991 Apr;72(4):508-13.
A variety of T-cell parameters have been analysed in two H-2 compatible strains of mice, B10.BR and BALB/K, which differ in an absolute fashion in their ability to resist infection with the parasitic nematode Trichuris muris: BALB/K mice expel T. muris relatively rapidly, whereas B10.BR mice are unable to expel the parasite before the infection reaches patency. Analysis of Th1- and Th2-specific cytokines (IFN-gamma and IL-5, respectively) produced by in vitro Con A-stimulated mesenteric lymph node cells (MLNC) from infected and normal mice demonstrated that MLNC from resistant BALB/K mice produced high levels of IL-5 and low levels of IFN-gamma whilst B10.BR MLNC secreted large amounts of IFN-gamma in the relative absence of IL-5. As an in vivo correlate of in vitro IL-5 production, peripheral and tissue eosinophilia were quantified during the course of infection in the two strains of mice. No peripheral eosinophilia was observed in BALB/K or B10.BR individuals. However, a considerable intestinal eosinophilia was seen in the high IL-5-producing BALB/K mice compared to normal levels. Differences observed in cytokine profiles were not due to differential changes in the numbers of T cells within the MLN. Indeed, FACS analysis revealed a decrease in the relative percentage of CD4+ and CD8+ T cells in both strains of mice post-infection. Our results suggest that resistance to T. muris involves the preferential induction of Th cells which secrete IL-5, whilst cells of a different Th subset (IFN-gamma producing) predominate in chronically infected mice. As such, this represents the first description of a correlation between the reciprocal activation of Th cell subsets in relation to acute or chronic intestinal infection with the same parasite in the same host species.
在两种H-2兼容的小鼠品系B10.BR和BALB/K中分析了多种T细胞参数，这两种品系在抵抗寄生线虫毛首鞭形线虫感染的能力上存在绝对差异：BALB/K小鼠相对较快地排出毛首鞭形线虫，而B10.BR小鼠在感染达到明显期之前无法排出寄生虫。对来自感染和正常小鼠的体外刀豆蛋白A刺激的肠系膜淋巴结细胞（MLNC）产生的Th1和Th2特异性细胞因子（分别为IFN-γ和IL-5）的分析表明，来自抗性BALB/K小鼠的MLNC产生高水平的IL-5和低水平的IFN-γ，而B10.BR MLNC在相对缺乏IL-5的情况下分泌大量的IFN-γ。作为体外IL-5产生的体内相关性，在两种小鼠品系的感染过程中对外周血和组织嗜酸性粒细胞进行了定量。在BALB/K或B10.BR个体中未观察到外周血嗜酸性粒细胞增多。然而，与正常水平相比，在产生高IL-5的BALB/K小鼠中观察到相当程度的肠道嗜酸性粒细胞增多。细胞因子谱中观察到的差异不是由于MLN内T细胞数量的差异变化。事实上，流式细胞术分析显示，两种小鼠品系感染后CD4 +和CD8 + T细胞的相对百分比均下降。我们的结果表明，对毛首鞭形线虫的抗性涉及优先诱导分泌IL-5的Th细胞，而不同的Th亚群（产生IFN-γ的细胞）在慢性感染的小鼠中占主导地位。因此，这首次描述了同一宿主物种中同一寄生虫急性或慢性肠道感染相关的Th细胞亚群相互激活之间的相关性。
