Salvemini M, Mauro U, Velaeti S, Polito C, Saccone G
Department of Biological Sciences, Section of Genetics and Molecular Biology, University Federico II, Naples, Italy.
Insect Mol Biol. 2006 Jun;15(3):341-9. doi: 10.1111/j.1365-2583.2006.00651.x.
The genetic transformation of insects by transposable elements is based on the use of selectable genetic markers required to identify transgenic individuals. Conserved regulatory sequences can be used to develop single constructs capable of adequate expression of a marker, across a range of different species. We present evidence that the Drosophila GBS regulatory element (Glass-binding site), derived from the Rh1 rhodopsin gene, is able to drive in vivo eye-specific expression of a Ccwhite+ transgene in the Mediterranean fruitfly Ceratitis capitata. The Ceratitis lineage diverged from that of Drosophila approximately 120 Myr ago. As the GBS regulatory sequence seems to be partially conserved in the more distantly related dipteran species Anopheles gambiae (250 Myr), we propose that the GBS may be widely useful for driving eye-specific expression in a wide range of dipteran species.
通过转座元件对昆虫进行基因转化是基于使用可选择的遗传标记来鉴定转基因个体。保守的调控序列可用于开发单一构建体，使其能够在一系列不同物种中充分表达标记物。我们提供的证据表明，源自Rh1视紫红质基因的果蝇GBS调控元件（玻璃结合位点）能够驱动地中海实蝇Ceratitis capitata体内Ccwhite +转基因的眼特异性表达。Ceratitis谱系与果蝇谱系大约在1.2亿年前分化。由于GBS调控序列似乎在更远缘的双翅目物种冈比亚按蚊（2.5亿年）中部分保守，我们提出GBS可能广泛用于在多种双翅目物种中驱动眼特异性表达。
