Zwiebel L J, Saccone G, Zacharopoulou A, Besansky N J, Favia G, Collins F H, Louis C, Kafatos F C
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Science. 1995 Dec 22;270(5244):2005-8. doi: 10.1126/science.270.5244.2005.
Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.
可靠的种系转化对于分子研究以及最终对经济上重要的昆虫（如地中海实蝇（medfly）Ceratitis capitata）的遗传控制是必需的。建立和维持转化株系的一个先决条件是可选择的或表型显性标记。为此，分离出了一个源自地中海实蝇白色基因的互补DNA克隆，它与黑腹果蝇和其他双翅目中的白色基因有很大的相似性。它与导致地中海实蝇白眼的自发突变相关，可用于恢复携带内源性白色基因无效突变的转基因果蝇的部分眼色。
