Ling Hua, Zhao Jingya, Zuo Kaijing, Qiu Chengxiang, Yao Hongyan, Qin Jie, Sun Xiaofen, Tang Kexuan
Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Key Laboratory of Agrobiotechnology, Shanghai Jiao Tong University, People's Republic of China.
J Biochem Mol Biol. 2006 May 31;39(3):297-303. doi: 10.5483/bmbrep.2006.39.3.297.
As lipolytic enzymes, GDSL lipases play an important role in plant growth and development. In order to identify their functions and roles, the full-length cDNA of a GDSL lipase gene, designated BnLIP2, was isolated from Brassica napus L. BnLIP2 was 1,300 bp long, with 1,122 bp open reading frame (ORF) encoding 373 amino acid residues. Sequence analysis indicated that BnLIP2 belonged to GDSL family. Southern blot analysis indicated that BnLIP2 belonged to a small gene family in rapeseed genome. RT-PCR analysis revealed that BnLIP2 was a tissue-specific expressing gene during reproductive growth and strongly expressed during seed germination. BnLIP2 expression could not be detected until three days after germination, and it subsequently became stronger. The transcript of this gene was deficient in root of seedlings growing at different stages. When juvenile seedlings were treated by methyl jasmonate (MeJ), salicylic acid (SA) and naphthalene acetic acid (NAA), BnLIP2 expression could not be induced in root. Our study implicates that BnLIP2 probably plays an important role in rapeseed germination, morphogenesis, flowering, but independent of root growth and development.
作为脂解酶，GDSL脂肪酶在植物生长发育中发挥着重要作用。为了鉴定它们的功能和作用，从甘蓝型油菜中分离出一个GDSL脂肪酶基因的全长cDNA，命名为BnLIP2。BnLIP2长1300 bp，具有1122 bp的开放阅读框（ORF），编码373个氨基酸残基。序列分析表明BnLIP2属于GDSL家族。Southern杂交分析表明BnLIP2属于油菜基因组中的一个小基因家族。RT-PCR分析显示BnLIP2是生殖生长过程中的一个组织特异性表达基因，在种子萌发时强烈表达。直到萌发三天后才能检测到BnLIP2的表达，随后表达增强。该基因的转录本在不同生长阶段的幼苗根中缺乏。当用茉莉酸甲酯（MeJ）、水杨酸（SA）和萘乙酸（NAA）处理幼苗时，根中不能诱导BnLIP2的表达。我们的研究表明，BnLIP2可能在油菜籽萌发、形态发生、开花中起重要作用，但与根的生长发育无关。
