College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China.
Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China.
Int J Mol Sci. 2023 Jun 19;24(12):10343. doi: 10.3390/ijms241210343.
GDSL esterases/lipases are a subclass of lipolytic enzymes that play critical roles in plant growth and development, stress response, and pathogen defense. However, the GDSL esterase/lipase genes involved in the pathogen response of apple remain to be identified and characterized. Thus, in this study, we aimed to analyze the phenotypic difference between the resistant variety, Fuji, and susceptible variety, Gala, during infection with , screen for anti-disease-associated proteins in Fuji leaves, and elucidate the underlying mechanisms. The results showed that GDSL esterase/lipase protein GELP1 contributed to infection defense in apple. During infection, expression was significantly upregulated in Fuji. Fuji leaves exhibited a highly resistant phenotype compared with Gala leaves. The formation of infection hyphae of was inhibited in Fuji. Moreover, recombinant His:GELP1 protein suppressed hyphal formation during infection in vitro. Transient expression in showed that GELP1-eGFP localized to the endoplasmic reticulum and chloroplasts. GELP1 overexpression in GL-3 plants increased resistance to . expression was upregulated in the transgenic lines. Notably, GELP1 transcript levels were elevated in GL-3 after salicylic acid treatment. These results suggest that GELP1 increases apple resistance to by indirectly regulating salicylic acid biosynthesis.
GDSL 酯酶/脂肪酶是脂肪酶的一个亚类，在植物生长发育、应激反应和病原体防御中起着关键作用。然而，参与苹果病原体反应的 GDSL 酯酶/脂肪酶基因仍有待鉴定和表征。因此，在本研究中，我们旨在分析富士和嘎拉在感染过程中表型的差异，筛选富士叶片中与抗病相关的蛋白质，并阐明其潜在机制。结果表明，GDSL 酯酶/脂肪酶蛋白 GELP1 有助于苹果对 的感染防御。在 感染过程中，GELP1 在富士中的表达显著上调。与嘎拉叶片相比，富士叶片表现出高度的抗性表型。感染菌丝的形成在富士中受到抑制。此外，重组 His:GELP1 蛋白在体外抑制菌丝形成。瞬时表达在 中表明 GELP1-eGFP 定位于内质网和叶绿体。在 GL-3 植物中过表达 GELP1 增加了对 的抗性。在转基因系中， 的表达上调。值得注意的是，在水杨酸处理后的 GL-3 中，GELP1 的转录本水平升高。这些结果表明，GELP1 通过间接调节水杨酸的生物合成增加了苹果对 的抗性。
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