Mättö J, Suihko M-L, Saarela M
VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Espoo, Finland.
Int J Antimicrob Agents. 2006 Jul;28(1):42-8. doi: 10.1016/j.ijantimicag.2006.02.010. Epub 2006 Jun 6.
The performance of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest was compared. All Bifidobacterium strains (n=42) displayed good growth on trypticase-phytone-yeast extract agar (TPY). Most strains showed good growth on lactic acid bacteria susceptibility test medium supplemented with cysteine (LSM+cys); Bifidobacterium bifidum showed moderate growth. Growth of seven strains was inadequate on Brucella blood agar (BRU) and an additional eight strains showed moderate growth. The minimum inhibitory concentrations (MICs) for tetracycline were highest on BRU and lowest on LSM+cys (agreement 57%), whereas the MICs for streptomycin were lowest on BRU and highest on TPY (agreement 40%). Occasional mismatches (agreement 71-91%) between the test media were also detected for the beta-lactam antibiotics. This study describes test medium-dependent variation of MICs and the applicability of LSM+cys for antimicrobial susceptibility testing of bifidobacteria.
比较了使用Etest对双歧杆菌进行抗菌药敏试验的三种测试培养基的性能。所有双歧杆菌菌株（n = 42）在胰蛋白酶-植物蛋白-酵母提取物琼脂（TPY）上生长良好。大多数菌株在添加半胱氨酸的乳酸菌药敏试验培养基（LSM + cys）上生长良好；两歧双歧杆菌生长中等。七株菌在布鲁氏菌血琼脂（BRU）上生长不足，另外八株菌生长中等。四环素的最低抑菌浓度（MIC）在BRU上最高，在LSM + cys上最低（一致率57%），而链霉素的MIC在BRU上最低，在TPY上最高（一致率40%）。对于β-内酰胺类抗生素，测试培养基之间也偶尔存在不匹配情况（一致率71 - 91%）。本研究描述了MIC的测试培养基依赖性变化以及LSM + cys在双歧杆菌抗菌药敏试验中的适用性。
