对来自密苏里州圣路易斯市一家儿童医院的耐甲氧西林金黄色葡萄球菌分离株进行基因分型时，重复序列聚合酶链反应与多位点序列分型的盲法比较。
Blinded comparison of repetitive-sequence PCR and multilocus sequence typing for genotyping methicillin-resistant Staphylococcus aureus isolates from a children's hospital in St. Louis, Missouri.
作者信息
Liao Robert S, Storch Gregory A, Buller Richard S, Orscheln Rachel C, Mardis Elaine R, Armstrong Jon R, Dunne W Michael
机构信息
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA.
出版信息
J Clin Microbiol. 2006 Jun;44(6):2254-7. doi: 10.1128/JCM.00690-06.
Abstract
We performed a blinded study to compare repetitive-sequence PCR and multilocus sequence typing for genotyping hospital- and community-acquired methicillin-resistant Staphylococcus aureus (MRSA). The MRSA strains that were sequence type 8 (ST8), staphylococcal cassette chromosome mec (SCCmec) type IV, and Panton-Valentine leukocidin-positive clustered separately from those that were ST5 and SCCmec type II.
摘要
我们进行了一项盲法研究，以比较重复序列聚合酶链反应（PCR）和多位点序列分型在医院获得性和社区获得性耐甲氧西林金黄色葡萄球菌（MRSA）基因分型中的应用。序列类型为8（ST8）、葡萄球菌盒式染色体mec（SCCmec）IV型且Panton-Valentine杀白细胞素阳性的MRSA菌株与序列类型为5和SCCmec II型的菌株分别聚类。
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