使用分子信标基于实时核酸序列扩增检测法检测1型人类免疫缺陷病毒的评估。
Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of human immunodeficiency virus type 1.
作者信息
McClernon D R, Vavro C, St Clair M
机构信息
Clinical Virology Department, GlaxoSmithKline, Research Triangle Park, NC 27709-3398, USA.
出版信息
J Clin Microbiol. 2006 Jun;44(6):2280-2. doi: 10.1128/JCM.02187-05.
Abstract
We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.
摘要
我们评估了一种新的基于核酸序列的实时扩增（NASBA）检测方法的性能特征，该方法采用分子信标技术检测1型人类免疫缺陷病毒（HIV-1）。定量结果与三种领先的市售检测方法所获得的结果相当。分析灵敏度为37 IU/ml。NASBA检测方法可检测临床相关的重组病毒以及所有M组HIV-1亚型。
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