Qutub Mohammed O, Klapper Paul E, Vallely Pam J, Cleator Gerham M, Mandall Daniel
Division of Virology, Department of Pathological Sciences, University of Manchester, United Kingdom.
Saudi Med J. 2006 Jun;27(6):788-93.
To asses the commercial available enzyme-linked immunosorbent assays (ELISA) for differentiation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) antibodies.
The study was performed between January 1997 to November 2002 in the Division of Virology, Department of Pathological Sciences, Central Manchester Healthcare Trust and University of Manchester, Manchester, United Kingdom. Assays based upon type-specific glycoprotein G-1 (gG-1) for HSV-1, and glycoprotein G-2 (gG-2) from HSV-2 were evaluated to differentiate between HSV-1 and HSV-2 antibodies. Using 5 different ELISA tests, 2 panels of serum samples were tested. Panel one consisted of 88 sera, selected from the serum bank of the Clinical Virology Laboratory, Manchester Royal Infirmary; panel 2 comprised of 90 sera selected from samples collected from Bangladeshi female commercial workers.
The data of this study showed that a high rate of gG-1 based immunoassays ranged from 87.9-100% for sensitivity and 51.5-100% specificity.
Although there are several immunoassays were claimed to differentiate between HSV-1 and HSV-2 antibodies, selection of these assays should be carefully interpreted with the overall clinical framework provided by detailed sexual history and genital examination.
评估用于区分1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）抗体的商用酶联免疫吸附测定（ELISA）。
该研究于1997年1月至2002年11月在英国曼彻斯特中央曼彻斯特医疗信托基金病理科学系病毒学部和曼彻斯特大学进行。对基于HSV-1的型特异性糖蛋白G-1（gG-1）和HSV-2的糖蛋白G-2（gG-2）的测定进行评估，以区分HSV-1和HSV-2抗体。使用5种不同的ELISA检测方法对2组血清样本进行检测。第一组由88份血清组成，选自曼彻斯特皇家医院临床病毒学实验室的血清库；第二组由90份血清组成，选自从孟加拉国女性商业工作者收集的样本。
本研究数据显示，基于gG-1的免疫测定灵敏度高，范围为87.9%至100%，特异性为51.5%至100%。
尽管有几种免疫测定方法声称可区分HSV-1和HSV-2抗体，但在详细的性病史和生殖器检查提供的整体临床框架下，应谨慎解读这些测定方法的选择。
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