Ramos Stephanie, Lukefahr James L, Morrow Rhoda Ashley, Stanberry Lawrence R, Rosenthal Susan L
Department of Pediatrics, University of Texas Medical Branch at Galveston, Galveston, TX 77555-0319, USA.
Pediatr Infect Dis J. 2006 Oct;25(10):902-5. doi: 10.1097/01.inf.0000237831.79072.c8.
Children and adolescents with a history of sexual abuse are at risk for acquiring herpes simplex virus (HSV) type 2. We evaluated the prevalence of HSV-1 and HSV-2 and the usefulness for this population of 2 commercially available tests.
Sera from 150 children seen in a sexual abuse clinic were analyzed for type-specific HSV antibodies using Focus HerpeSelect HSV-2 ELISA (Focus), Biokit HSV-2 Rapid Test (Biokit), and by Western blot (WB).
The patient sample was 81% female, had a mean age of 11.6 years (range, 1 to 18 years), and was 46% Caucasian, 28% Hispanic, 25% African American, and 2% other. According to WB, 77 (51%) of the children were HSV-1 seropositive. For HSV-2, there was 1 "true positive" (positive by all 3 tests) and 1 patient whose serum had atypical HSV-2 bands by WB but was positive by Focus. There were 6 sera that were positive by Focus and negative by WB. Index values of these 6 Focus tests were not predictive of WB status. For the 105 samples for which Biokit data were available, all samples were concordant with WB.
The findings of this study suggest that routine screening for HSV-2 in sexually abused children does not have a high yield. The Focus test has an unacceptably high rate of false-positive results in children; however, Biokit may be an acceptable substitute for WB in evaluating children for HSV-2 antibodies.
有性虐待史的儿童和青少年感染2型单纯疱疹病毒（HSV）的风险较高。我们评估了HSV-1和HSV-2的流行情况以及两种市售检测方法对该人群的实用性。
对一家性虐待诊所的150名儿童的血清进行分析，使用Focus HerpeSelect HSV-2 ELISA（Focus）、Biokit HSV-2快速检测法（Biokit）和免疫印迹法（WB）检测型特异性HSV抗体。
患者样本中81%为女性，平均年龄11.6岁（范围1至18岁），46%为白种人，28%为西班牙裔，25%为非裔美国人，2%为其他种族。根据WB检测，77名（51%）儿童HSV-1血清学呈阳性。对于HSV-2，有1例“真阳性”（所有3种检测均为阳性），1例患者血清通过WB检测有非典型HSV-2条带，但Focus检测为阳性。有6份血清Focus检测为阳性而WB检测为阴性。这6次Focus检测的指数值无法预测WB检测结果。对于有Biokit数据的105份样本，所有样本与WB检测结果一致。
本研究结果表明，对性虐待儿童进行HSV-2常规筛查的阳性率不高。Focus检测在儿童中的假阳性率高得令人无法接受；然而，在评估儿童HSV-2抗体时，Biokit可能是WB检测的可接受替代方法。
