用于测定抗单纯疱疹病毒1型和2型型特异性IgG抗体的新型重组酶联免疫吸附测定法。

Novel recombinant ELISA assays for determination of type-specific IgG antibodies against HSV-1 and HSV-2.

作者信息

Sauerbrei Andreas, Wutzler Peter

机构信息

Institute of Virology and Antiviral Therapy, Friedrich-Schiller University, Hans-Knoell-Strasse 2, D-07745 Jena, Germany.

出版信息

J Virol Methods. 2007 Sep;144(1-2):138-42. doi: 10.1016/j.jviromet.2007.04.007. Epub 2007 Jun 4.

DOI:10.1016/j.jviromet.2007.04.007

PMID:17548119

Abstract

The two novel Novagnost enzyme-linked immunosorbent assays (ELISA, Dade Behring) based on recombinant viral glycoprotein G were evaluated for determination of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) IgG. ELISA and immunoblot procedures approved by the US Food and Drug Administration served as the reference for selecting different serum panels to be tested. The sensitivity and specificity of the novel assay for HSV-1 IgG were 93.1-98.0% and 99.3-100%, respectively, while the sensitivity and specificity of the novel assay for HSV-2 IgG were 100% and 94.6-97.6%, respectively. Using Kappa statistics, the Kappa indices were computed at 0.93-0.98 for the HSV-1 assays and 0.92-0.96 for the HSV-2 assays suggesting an almost perfect agreement between the novel ELISAs and the reference tests. In conclusion, the novel immunoassays for determination of HSV-1 or HSV-2 IgG can be recommended for the reliable type-specific diagnosis of HSV infections.

摘要

对两种基于重组病毒糖蛋白G的新型诺瓦格诺斯酶联免疫吸附测定法（ELISA，达德拜林公司）进行了评估，以测定1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）的IgG。美国食品药品监督管理局批准的ELISA和免疫印迹程序用作选择不同待测血清样本的参考标准。新型检测法检测HSV-1 IgG的灵敏度和特异性分别为93.1%-98.0%和99.3%-100%，而检测HSV-2 IgG的灵敏度和特异性分别为100%和94.6%-97.6%。使用卡方统计，HSV-1检测的卡方指数计算结果为0.93-0.98，HSV-2检测的卡方指数为0.92-0.96，这表明新型ELISA与参考检测之间几乎完全一致。总之，用于测定HSV-1或HSV-2 IgG的新型免疫测定法可推荐用于HSV感染的可靠型特异性诊断。