Brack W, Frank H
Department of Environmental Chemistry and Ecotoxicology, University of Bayreuth, Germany.
Ecotoxicol Environ Saf. 1998 May-Jun;40(1-2):34-41. doi: 10.1006/eesa.1997.1639.
The effects of environmental pollutants, such as triazines, urea herbicides, phenols, nitro aromatic compounds, aldehydes, hydrogen sulfide, volatile halogenated aliphatic and aromatic compounds, and alkylbenzenes, the photosynthesis of green algae were investigated by using detailed analysis of chlorophyll a fluorescence. Application of quenching analysis by means of a pulse amplitude-modulated fluorometer allows the discrimination of different modes of action. Blocks of the electron-transport chain, the action of uncouplers, and fluorescence quenching by nitroaormatic compounds could be distinguished. Hydrogen sulfide and short-chain aldehydes seem to react in the same way, probably by reaction with components of the electron-transport chain. Nonreactive volatile compounds affect chlorophyll fluorescence in a strictly hydrophobicity-dependent manner. The quenching analysis suggests an inhibition of utilization of proton-motive force for ATP synthesis. Detailed analysis of fluorescence patterns may be of great help for identification of toxic pollutants and their sources.
通过对叶绿素a荧光进行详细分析，研究了三嗪、尿素除草剂、酚类、硝基芳香化合物、醛类、硫化氢、挥发性卤代脂肪族和芳香族化合物以及烷基苯等环境污染物对绿藻光合作用的影响。使用脉冲幅度调制荧光计进行猝灭分析，能够区分不同的作用模式。可以区分电子传递链的阻断、解偶联剂的作用以及硝基芳香化合物引起的荧光猝灭。硫化氢和短链醛类似乎以相同的方式反应，可能是与电子传递链的成分发生反应。非反应性挥发性化合物以严格依赖疏水性的方式影响叶绿素荧光。猝灭分析表明质子动力用于ATP合成的过程受到抑制。对荧光模式的详细分析可能对识别有毒污染物及其来源有很大帮助。
