Stiller R A, Belamarich F A, Shepro D
Am J Physiol. 1975 Jul;229(1):206-10. doi: 10.1152/ajplegacy.1975.229.1.206.
Avian thromboyctes are aggregated by a number of substances that cause platelet aggregation, and evidence suggests that this response is related to the release of serotonin (5-hydroxytryptamine, 5-HT) from intracellular granules. In this study duck thrombocytes released 5-HT during collagen-induced aggregation, but thrombocytes incubated with 14C-labeled adenine did not release radioactive adenine nucleotides. These results indicate the existence of a metabolic pool of adenine nucleotides that is separate from released constituents of the cell. No unlabeled adenine compounds were detected in the supernatants of aggregated thrombocytes indicating either the rapid alteration of released nucleotides or the absence of a specific release pool of adenine nucleotides. Finally there is no release of the intracellular enzyme markers, lactate dehydrogenase, beta-glucuronidase, and acid phosphatase, during collagen-induced aggregation. These findings suggest that avian thrombocytes exhibit a specific release reaction and that serotonin acts as the functional counterpart of ADP in platelet aggregation.
禽类血小板可被多种能引起血小板聚集的物质所聚集，有证据表明这种反应与细胞内颗粒中血清素（5-羟色胺，5-HT）的释放有关。在本研究中，鸭血小板在胶原诱导的聚集过程中释放5-HT，但与14C标记腺嘌呤孵育的血小板并未释放放射性腺嘌呤核苷酸。这些结果表明存在一个与细胞释放成分相分离的腺嘌呤核苷酸代谢池。在聚集血小板的上清液中未检测到未标记的腺嘌呤化合物，这表明释放的核苷酸迅速发生了变化，或者不存在特定的腺嘌呤核苷酸释放池。最后，在胶原诱导的聚集过程中，细胞内酶标记物乳酸脱氢酶、β-葡萄糖醛酸酶和酸性磷酸酶并未释放。这些发现表明禽类血小板表现出特异性释放反应，且血清素在血小板聚集中充当ADP的功能对应物。
