Yu Chao-hui, Li Lan, Li You-ming, Zhang Bao-feng, Fang Jing, Zhou Qiong, Hu Ying, Gao Heng-jun
Department of Gastroenterology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Zhonghua Nei Ke Za Zhi. 2006 Aug;45(8):658-60.
To constructed tissue microarray containing specimens from gastric cancer and adjacent non-cancer tissue to survey the expression of p53, p16 and cyclooxygenase-2 (COX-2), and to obtain a comprehensive survey on the expression of three proteins in gastric cancer progression and their clinical significance.
We constructed a tissue microarray containing 50 specimens from gastric cancer and 78 specimens from adjacent non-cancer tissue, and assayed three different proteins (p53, p16 and COX-2) by immunohistochemistry to consecutive formalin-fixed tissue microarray sections.
In non-cancer tissue, p53, p16 and COX-2 positive staining were 19%, 15% and 74% respectively. In gastric cancer tissue, p53, p16 and COX-2 positive staining were 50%, 54% and 94% respectively. There were a significant difference between two different tissues (P < 0.05). 52% (66/128) cases were COX-2+/p53-, only 1 case were COX-2-/p53+. There was a significant association between COX-2 and p53 (P < 0.05). COX-2 negative expression tissues were found significantly more often in p53-negative than in p53-positive cases. 52% (67/128) cases were COX-2+/p16-, only 1 case were COX-2-/p16+. A significant correlation was recognized between expression of COX-2 and p16 (P < 0.05). Logistic regression analysis revealed that the risk factors of tumorous histotype were p53, p16 and COX-2 positive expression together, determined by Wald chi2 test (P < 0.05; odds ratio, 18.889). There was reciprocal relationship among p53, p16 and COX-2.
Combination analysis of p53, p16 and COX-2 may be useful for the prediction of gastric carcinogenesis.
构建包含胃癌及癌旁非癌组织标本的组织芯片，检测p53、p16和环氧化酶-2（COX-2）的表达，全面了解这三种蛋白在胃癌进展中的表达情况及其临床意义。
构建包含50例胃癌标本和78例癌旁非癌组织标本的组织芯片，采用免疫组织化学方法对连续的福尔马林固定组织芯片切片检测三种不同蛋白（p53、p16和COX-2）。
在非癌组织中，p53、p16和COX-2阳性染色分别为19%、15%和74%。在胃癌组织中，p53、p16和COX-2阳性染色分别为50%、54%和94%。两种不同组织之间存在显著差异（P<0.05）。52%（66/128）的病例为COX-2+/p53-，仅1例为COX-2-/p53+。COX-2与p53之间存在显著关联（P<0.05）。在p53阴性病例中，COX-2阴性表达组织的出现频率明显高于p53阳性病例。52%（67/128）的病例为COX-2+/p16-，仅1例为COX-2-/p16+。COX-2与p16的表达之间存在显著相关性（P<0.05）。Logistic回归分析显示，肿瘤组织学类型的危险因素为p53、p16和COX-2共同阳性表达，经Wald卡方检验确定（P<0.05；优势比，18.889）。p53、p16和COX-2之间存在相互关系。
p53、p16和COX-2的联合分析可能有助于预测胃癌的发生。
