[H3亚型流感病毒流行株对金刚烷胺的耐药性：初步研究]
[Resistance to amantadine of epidemic strain of H3 subtype influenza virus: a primary study].
作者信息
Xu Hong, Qian Yuan, Xu Hang, Zhang Jia-huai, Zhu Ru-nan, Wang Fang
机构信息
Institute for Viral Disease Control and Prevention, China Centre for Disease Control and Prevention, Beijing 100052, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2006 Apr 11;86(14):996-8.
OBJECTIVE
To study the resistance to amantadine of epidemic strain of H3 subtype influenza virus.
METHODS
Forty-one strains of influenza virus were isolated from pediatric patients with influenza in Beijing 2004-2005 and were identified as H3 subtype. RNA was extracted. Thirty of the 40 strains were resistant to amantadine with a resistance rate of 75%. The M2 gene ion channel fragments were amplified by one-step RT-PCR, sequenced, and then underwent systemic tree analysis.
RESULTS
M2 ion channel associated gene fragment with a molecular size of 153 bp was obtained. The amino acid at the site 31 changed from serine into asparagines, a mutation identical to that of the reference strain A/PR8/34 resistant to amantadine.
CONCLUSION
The resistance rate to amantadine is high among the epidemic strains of H3 subtype influenza virus in China. The associated mutation occurs at the site 31 of M2 protein.
目的
研究H3亚型流感病毒流行株对金刚烷胺的耐药性。
方法
2004 - 2005年从北京流感患儿中分离出41株流感病毒，鉴定为H3亚型。提取RNA。40株中有30株对金刚烷胺耐药，耐药率为75%。采用一步法RT-PCR扩增M2基因离子通道片段，测序，然后进行系统进化树分析。
结果
获得了分子大小为153 bp的M2离子通道相关基因片段。第31位氨基酸由丝氨酸变为天冬酰胺，该突变与对金刚烷胺耐药的参考株A/PR8/34相同。
结论
我国H3亚型流感病毒流行株对金刚烷胺的耐药率较高。相关突变发生在M2蛋白的第31位。
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