Higgins Rachel R, Eshaghi A, Burton L, Mazzulli T, Drews S J
Central Public Health Laboratory, Toronto, ON, Canada.
J Clin Virol. 2009 Jan;44(1):91-3. doi: 10.1016/j.jcv.2008.10.001. Epub 2008 Nov 18.
Molecular methods were used to characterize influenza A (H1N1) and (H3N2) strains and to identify amantadine-resistance.
To compare proportions of amantadine-resistant influenza A (H1N1) and (H3N2) isolates in the Greater Toronto Area.
Isolates of influenza A (H1N1) and (H3N2) were strain typed using molecular methods. Pyrosequencing for point mutations in the transmembrane domain of the M2 proton channel was undertaken. Proportions of amantadine-resistant and susceptible isolates were compared using the The Fisher's exact test.
96% of the 49 influenza A (H3N2) isolates and none of the influenza A (H1N1) tested carried a point mutation in the M gene coding for the M2 protein. Influenza A (H3N2) isolates were more likely to carry an amantadine-resistance associated mutation than influenza A (H1N1) isolates (Fishers's exact test, P<0.0001).
: Characterization of amantadine-resistance in influenza A (H1N1) isolates should utilize a variety of different methods including sub-typing, strain typing, and direct sequencing for point mutations associated with amantadine-resistance.
采用分子方法对甲型流感（H1N1）和（H3N2）毒株进行特征分析，并鉴定金刚烷胺耐药性。
比较大多伦多地区甲型流感（H1N1）和（H3N2）分离株中金刚烷胺耐药株的比例。
采用分子方法对甲型流感（H1N1）和（H3N2）分离株进行毒株分型。对M2质子通道跨膜结构域的点突变进行焦磷酸测序。采用Fisher精确检验比较金刚烷胺耐药株和敏感株的比例。
49株甲型流感（H3N2）分离株中有96%以及所检测的甲型流感（H1N1）分离株中均无M2蛋白编码M基因的点突变。甲型流感（H3N2）分离株比甲型流感（H1N1）分离株更易携带与金刚烷胺耐药相关的突变（Fisher精确检验，P<0.0001）。
对甲型流感（H1N1）分离株的金刚烷胺耐药性进行特征分析时，应采用多种不同方法，包括亚型分型、毒株分型以及对与金刚烷胺耐药相关的点突变进行直接测序。
