Stadejek T, Oleksiewicz M B, Potapchuk D, Podgórska K
National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland.
Novo Nordisk A/S, Virology and Molecular Toxicology, Novo Nordisk Park, 2760 Måløv, Denmark.
J Gen Virol. 2006 Jul;87(Pt 7):1835-1841. doi: 10.1099/vir.0.81782-0.
Porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 and ORF7 sequences from Belarus were found to be of the European (EU) genotype, but grouped separately from all other EU genotype sequences described so far, including live-attenuated EU genotype PRRSV vaccines and Italian EU genotype sequences, some of which have been associated with reduced vaccine efficacy. Also, the Belarusian EU-PRRSV exhibited extreme ORF7 size polymorphism, ranging from 375 nt (the smallest EU genotype ORF7 yet described) to 393 nt (the largest ORF7 yet described for any arterivirus). With the Belarusian sequences, the diversity of EU genotype PRRSV now exceeds that of the North American (US) genotype PRRSV, suggesting a European origin of PRRSV. Finally, a very sharp geographical demarcation of highly diverse EU genotype PRRSV was observed along the eastern Polish border. The new Belarusian sequences have relevance for vaccine and diagnostic-antigen design and show that sequence analysis of PRRSV from more eastern parts of Europe may offer further insights into the emergence and evolution of PRRSV.
来自白俄罗斯的猪繁殖与呼吸综合征病毒（PRRSV）的ORF5和ORF7序列被发现属于欧洲（EU）基因型，但与迄今描述的所有其他EU基因型序列分开聚类，包括减毒活EU基因型PRRSV疫苗和意大利EU基因型序列，其中一些与疫苗效力降低有关。此外，白俄罗斯的EU-PRRSV表现出极端的ORF7大小多态性，范围从375个核苷酸（迄今描述的最小的EU基因型ORF7）到393个核苷酸（迄今为任何动脉炎病毒描述的最大的ORF7）。有了白俄罗斯的序列，EU基因型PRRSV的多样性现在超过了北美（US）基因型PRRSV，这表明PRRSV起源于欧洲。最后，在波兰东部边境沿线观察到高度多样的EU基因型PRRSV有非常明显的地理划分。新的白俄罗斯序列与疫苗和诊断抗原设计相关，并表明对来自欧洲更东部地区的PRRSV进行序列分析可能会为PRRSV的出现和进化提供进一步的见解。
