Soos T J, Sims T N, Barisoni L, Lin K, Littman D R, Dustin M L, Nelson P J
Division of Nephrology, New York University School of Medicine, New York, New York 10016, USA.
Kidney Int. 2006 Aug;70(3):591-6. doi: 10.1038/sj.ki.5001567. Epub 2006 Jun 7.
Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.
树突状细胞（DCs）在非淋巴器官中连接天然免疫和适应性免疫；然而，肾脏内DC的确切分布和类型尚不清楚。我们利用CX3CR1GFP/+小鼠来表征正常肾脏中组织驻留CX3CR1+ DC的解剖结构和表型。激光扫描共聚焦显微镜显示，在整个肾脏的间质和系膜空间中，有广泛的、连续的星状CX3CR1+ DC网络。浅表皮质的活体显微镜检查显示，静止的间质CX3CR1+ DC通过树突延伸不断探测周围组织环境。肾脏CX3CR1+ DC的流式细胞术显示，CD11c和F4/80有显著共表达，主要组织相容性复合体II类和FcR表达高，共刺激不成熟但吞噬能力良好，表明是准备对环境线索作出反应的组织驻留、不成熟DC。因此，在肾实质内，肾脏CX3CR1+ DC提供的监视几乎不存在免疫豁免，CX3CR1+ DC是构成正常肾脏的异质性单核吞噬细胞系统的主要成分。
