Honour John W
Department of Clinical Biochemistry, University College London Hospitals, UK.
Methods Mol Biol. 2006;324:53-74. doi: 10.1385/1-59259-986-9:53.
Gas chromatography-mass spectrometry (GC-MS) has been used in hormone assays particularly for steroids in biological fluids. The combination of GC with MS exploits the high-resolving power of gas chromatography to separate closely related molecules, and the ability of the MS to provide precise data for identification and quantification of the separated substances. GC-MS is a very powerful technique for analysis with specificity of hormones in biological fluids. The general principles of GC-MS are described in this chapter along with some examples that illustrate specific applications of hormone analysis.
气相色谱-质谱联用（GC-MS）已用于激素检测，特别是生物体液中类固醇激素的检测。气相色谱与质谱联用发挥了气相色谱的高分辨率，可分离密切相关的分子，同时质谱能够为已分离物质的鉴定和定量提供精确数据。GC-MS是一种非常强大的分析技术，可特异性分析生物体液中的激素。本章将介绍GC-MS的一般原理，并举例说明激素分析的具体应用。
