肿瘤相关抗原RCAS1在口腔鳞状细胞癌中的表达及其可能参与免疫逃逸的机制
Expression of tumor-associated antigen RCAS1 and its possible involvement in immune evasion in oral squamous cell carcinoma.
作者信息
Toyoshima T, Nakamura S, Kumamaru W, Kawamura E, Ishibashi H, Hayashida J-N, Moriyama M, Ohyama Y, Sasaki M, Shirasuna K
机构信息
Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
出版信息
J Oral Pathol Med. 2006 Jul;35(6):361-8. doi: 10.1111/j.1600-0714.2006.00442.x.
BACKGROUND
RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is known to induce apoptosis in its receptor-positive cells. The authors investigated RCAS1 expression in oral squamous cell carcinoma (SCC) and its association with the apoptosis of tumor-infiltrating lymphocytes (TILs).
METHODS
In 130 patients with oral SCC, the expression of RCAS1 in tumor cells was immunohistochemically examined and the apoptosis of TILs was examined by Terminal Deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) staining.
RESULTS
RCAS1 was detected both on the cytoplasm and the membrane of tumor cells in 41 of 130 cases (31.5%). Focusing on the expression at the invasive front interacting with host immune cells, RCAS1 was detected in 22 of 130 cases (16.9%). The percentage of TUNEL-positive TILs in cases with RCAS1-positive SCCs was significantly higher than in cases with RCAS1-negative SCCs (P < 0.0001).
CONCLUSIONS
RCAS1 can be expressed on oral SCC cells and may be involved in the tumor escape from the host immune system by inducing the apoptosis of TILs.
背景
已知RCAS1（SiSo细胞上表达的受体结合癌抗原）可诱导其受体阳性细胞发生凋亡。作者研究了口腔鳞状细胞癌（SCC）中RCAS1的表达及其与肿瘤浸润淋巴细胞（TILs）凋亡的关系。
方法
对130例口腔SCC患者，采用免疫组织化学方法检测肿瘤细胞中RCAS1的表达，并用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测TILs的凋亡情况。
结果
130例中有41例（31.5%）肿瘤细胞的细胞质和细胞膜上均检测到RCAS1。聚焦于与宿主免疫细胞相互作用的浸润前沿的表达，130例中有22例（16.9%）检测到RCAS1。RCAS1阳性SCC病例中TUNEL阳性TILs的百分比显著高于RCAS1阴性SCC病例（P < 0.0001）。
结论
RCAS1可在口腔SCC细胞上表达，并可能通过诱导TILs凋亡参与肿瘤逃避宿主免疫系统的过程。
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