Dash Kailash C, Mahapatra Niva, Bhuyan Lipsa, Panda Abikshyeet, Behura Shyam S, Mishra Pallavi
Department of Oral Pathology and Microbiology, Kalinga Institute of Dental Sciences, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, India.
J Pharm Bioallied Sci. 2020 Aug;12(Suppl 1):S274-S278. doi: 10.4103/jpbs.JPBS_83_20. Epub 2020 Aug 28.
Ki-67 is a nuclear protein. It is a proliferation marker that has an essential function in tumorigenesis due to its positive connection with tumor expansion.
The aim of this study was to evaluate the articulation of Ki‑67 as prognostic marker in various grades of oral epithelial dysplasia (OED) and in oral squamous cell carcinoma (OSCC).
A total of 100 histologically affirmed samples of normal oral mucosa (NOM), OED, and OSCC were divided into three groups-Group I (10 samples of normal oral mucosa), Group II (45 samples of OED), Group III (45 samples of OSCC). Routine hematoxylin and eosin and immunohistochemical staining with Ki-67 monoclonal antibody were carried out in all the samples.
Within Group I, articulation of Ki-67 was constrained to the basal layers. In Group II, cells showing positive expression of Ki-67 were available in the basal, suprabasal, and spinous layers. Cells showing positive expression of Ki-67 among well-differentiated OSCC were presented mainly in the periphery of the tumor nests; in moderately differentiated OSCC, cells were located in both peripheral and part of a center of the tumor nests; and in most cases of poorly differentiated OSCC, cells were diffused. Statistically significant difference in positive expression of Ki-67 was appreciated between three groups.
Ki-67 antigen may perhaps be used as a marker for the histological reviewing of OED and OSCC. With the increase in the severity of OED, cells showing positive expression of Ki-67 also increased.
Ki-67是一种核蛋白。它是一种增殖标志物，由于其与肿瘤生长呈正相关，在肿瘤发生中具有重要作用。
本研究旨在评估Ki-67作为不同分级口腔上皮发育异常（OED）和口腔鳞状细胞癌（OSCC）预后标志物的表达情况。
总共100例经组织学证实的正常口腔黏膜（NOM）、OED和OSCC样本被分为三组——第一组（10例正常口腔黏膜样本）、第二组（45例OED样本）、第三组（45例OSCC样本）。对所有样本进行常规苏木精和伊红染色以及Ki-67单克隆抗体免疫组化染色。
在第一组中，Ki-67的表达局限于基底层。在第二组中，Ki-67呈阳性表达的细胞见于基底层、基底上层和棘层。在高分化OSCC中，Ki-67呈阳性表达的细胞主要出现在肿瘤巢的周边；在中分化OSCC中，细胞位于肿瘤巢的周边和部分中心；在大多数低分化OSCC病例中，细胞呈弥漫分布。三组之间Ki-67阳性表达的差异具有统计学意义。
Ki-67抗原可能可作为OED和OSCC组织学评估的标志物。随着OED严重程度的增加，Ki-67呈阳性表达的细胞也增多。
