Daniels S, Zhao D M, Inman N, Price D J, Shelton C J, Smith E B
Physical Chemistry Laboratory, Oxford, England.
Ann N Y Acad Sci. 1991;625:108-15. doi: 10.1111/j.1749-6632.1991.tb33834.x.
The effects of general anesthetics and pressure on receptors from the mammalian central nervous system have been investigated using oocyte expression techniques. Poly A+ mRNA extracted from rat whole brain was injected into mature Xenopus oocytes producing depolarizing responses to the fast excitatory neurotransmitters NMDA and kainate and the inhibitory neurotransmitters GABA and glycine. An apparatus was constructed to allow agonist dose-response curves to be determined at high pressures using voltage-clamped oocytes. This was used to investigate the excitatory transmitter kainate. It was found that anesthetics depress the current induced by kainate whereas pressure does not appear to affect the responses associated with this transmitter. Furthermore it was found that pressure does not reverse (or modify in any way) the changes in response brought about by application of anesthetics.
利用卵母细胞表达技术研究了全身麻醉剂和压力对哺乳动物中枢神经系统受体的影响。从大鼠全脑中提取的多聚腺苷酸+信使核糖核酸被注入成熟的非洲爪蟾卵母细胞中，这些卵母细胞对快速兴奋性神经递质N-甲基-D-天冬氨酸（NMDA）和海人酸以及抑制性神经递质γ-氨基丁酸（GABA）和甘氨酸产生去极化反应。构建了一种装置，以便使用电压钳制的卵母细胞在高压下测定激动剂剂量反应曲线。该装置用于研究兴奋性递质海人酸。结果发现，麻醉剂会抑制海人酸诱导的电流，而压力似乎不会影响与该递质相关的反应。此外，还发现压力不会逆转（或以任何方式改变）麻醉剂应用所引起的反应变化。
