在注射了小鼠脑信使核糖核酸的非洲爪蟾卵母细胞中，牛磺酸和β-丙氨酸作用于γ-氨基丁酸（GABA）和甘氨酸受体。

Taurine and beta-alanine act on both GABA and glycine receptors in Xenopus oocyte injected with mouse brain messenger RNA.

作者信息

Horikoshi T, Asanuma A, Yanagisawa K, Anzai K, Goto S

机构信息

Department of Physiology, School of Dental Medicine, Tsurumi University, Yokohama, Japan.

出版信息

Brain Res. 1988 Sep;464(2):97-105. doi: 10.1016/0169-328x(88)90002-2.

DOI:10.1016/0169-328x(88)90002-2

PMID:2464409

Abstract

The responding pathway (process from agonist binding to channel opening) of taurine and beta-alanine was investigated in Xenopus oocytes injected with mouse brain poly(A)+ RNA. Responses to gamma-aminobutyric acid (GABA), glycine, taurine and beta-alanine were induced in oocytes injected with poly(A)+ RNA extracted from 3 regions, cerebrum, cerebellum and brainstem of the mouse brain. From comparison, responses to these 4 inhibitory amino acids in each regional poly(A)+ RNA-injected oocytes were categorized into at least 3 groups: (1) GABA, (2) glycine, and (3) taurine and beta-alanine. No cross-desensitization was observed between GABA response and glycine response, but taurine and beta-alanine responses cross-desensitized both the GABA and glycine responses. Taurine and beta-alanine responses were partially inhibited by the GABA antagonist, bicuculline, and also by the glycine antagonist, strychnine. The results suggest that the taurine or the beta-alanine response in the brain is caused through both the GABA receptor and the glycine receptor.

摘要

在注射了小鼠脑 poly(A)+ RNA 的非洲爪蟾卵母细胞中，研究了牛磺酸和 β-丙氨酸的反应途径（从激动剂结合到通道开放的过程）。在用从小鼠脑的 3 个区域（大脑、小脑和脑干）提取的 poly(A)+ RNA 注射的卵母细胞中，诱导出了对γ-氨基丁酸（GABA）、甘氨酸、牛磺酸和 β-丙氨酸的反应。通过比较，在每个区域注射 poly(A)+ RNA 的卵母细胞中，对这 4 种抑制性氨基酸的反应至少分为 3 组：（1）GABA，（2）甘氨酸，（3）牛磺酸和 β-丙氨酸。在 GABA 反应和甘氨酸反应之间未观察到交叉脱敏，但牛磺酸和 β-丙氨酸反应使 GABA 和甘氨酸反应均发生交叉脱敏。牛磺酸和 β-丙氨酸反应部分受到 GABA 拮抗剂荷包牡丹碱以及甘氨酸拮抗剂士的宁的抑制。结果表明，脑中牛磺酸或 β-丙氨酸反应是通过 GABA 受体和甘氨酸受体两者引起的。