Venet A, Lu W, Beldjord K, Andrieu J M
Laennec HIV Study Group, Hôpital Laennec, Paris, France.
AIDS. 1991 Mar;5(3):283-8. doi: 10.1097/00002030-199103000-00006.
We conducted a study of 152 HIV-1-seropositive individuals in order to evaluate the possible correlations between the isolation of HIV from peripheral blood mononuclear cells or from plasma and CD4 cell counts. HIV was isolated from only 36% of plasma samples, and the isolation rate was closely related to CD4 cell counts, increasing gradually from 0% in subjects with greater than 800 x 10(6)/l CD4 cells to 88% in those with less than 100 x 10(6)/l CD4 cells. In contrast, HIV was isolated from 92% of cell samples (99% in subjects with less than 900 x 10(6)/l CD4 cells, 46% in those with CD4 counts greater than or equal to 900 x 10(6)/l). Since most cell samples were positive, a scoring method was designed to quantify the cellular viral load. The results obtained demonstrated that the cellular viral load was closely related to CD4 counts. We also found that the cellular viral load was higher in subjects with either positive plasma isolation or positive p24 antigenaemia. The measurement of the cellular viral load by this scoring method appears to be useful for the management of HIV-seropositive individuals and for the evaluation of therapeutic trials.
我们对152名HIV-1血清反应阳性个体进行了一项研究，以评估从外周血单个核细胞或血浆中分离出HIV与CD4细胞计数之间可能存在的相关性。仅从36%的血浆样本中分离出了HIV，且分离率与CD4细胞计数密切相关，从CD4细胞计数大于800×10⁶/l的受试者中的0%逐渐增至CD4细胞计数小于100×10⁶/l的受试者中的88%。相比之下，从92%的细胞样本中分离出了HIV（CD4细胞计数小于900×10⁶/l的受试者中为99%，CD4细胞计数大于或等于900×10⁶/l的受试者中为46%）。由于大多数细胞样本呈阳性，因此设计了一种评分方法来量化细胞病毒载量。所获得的结果表明，细胞病毒载量与CD4计数密切相关。我们还发现，血浆分离阳性或p24抗原血症阳性的受试者的细胞病毒载量更高。通过这种评分方法测量细胞病毒载量似乎有助于对HIV血清反应阳性个体进行管理以及评估治疗试验。
