Kanat Ozkan, Kurt E, Yalcinkaya U, Evrensel T, Manavoglu O
Uludag University, Faculty of Medicine, Department of Medical Oncology, Gorukle 16059, Bursa, Turkey.
Indian J Cancer. 2006 Jan-Mar;43(1):12-5. doi: 10.4103/0019-509x.25769.
Hemorrhagic cystitis (HC) is a dose limiting side effect of cyclophosphamide (CYP).
In this study, we aimed to investigate the role of amifostine in the protection of CYP-induced HC and compare its efficacy with mesna.
This animal study was conducted in the Experimental Animals Breeding and Research Center of the Medical Faculty of Uludag University.
Male Wistar rats (150-200 g; 10 rats per group) were randomly assigned to four groups. Group I (control group) received no drugs, group II received CYP (200 mg/kg, i.p.) alone, group III received amifostine (200 mg/kg, i.p.) and CYP, and group IV received CYP and mesna (40 mg/kg, i.p.) immediately and 4 and 8 h after administration of CYP. Bladders of animals were assessed macroscopically and histologically 24 h later. Gross assessment for presence of edema and hemorrhage and histological evaluation of damage to the bladder were scored according to Gray's criteria.
For macroscopic and microscopic data, we used statistical evaluation by Kruskal-Wallis nonparametric analysis of variance followed by the Mann-Whitney U-test.
All the animals in group II had evidence of HC. Significant histological damage and macroscopic changes were present in this group compared to control group (P<0.001). The median scores for bladder damage in group III and IV were significantly lower compared to group II (P<0.001). When the median scores for bladder damage of group I, III, and IV were compared, there was no significant difference among these groups.
This study demonstrated the efficacy of amifostine in prevention of cyclophosphamide-induced hemorrhagic cystitis.
出血性膀胱炎（HC）是环磷酰胺（CYP）的剂量限制性副作用。
在本研究中，我们旨在探讨氨磷汀在保护CYP诱导的HC中的作用，并将其疗效与美司钠进行比较。
本动物研究在乌鲁达格大学医学院实验动物饲养与研究中心进行。
雄性Wistar大鼠（150 - 200克；每组10只大鼠）随机分为四组。第一组（对照组）不接受药物，第二组单独接受CYP（200毫克/千克，腹腔注射），第三组接受氨磷汀（200毫克/千克，腹腔注射）和CYP，第四组在给予CYP后立即以及4小时和8小时接受CYP和美司钠（40毫克/千克，腹腔注射）。24小时后对动物的膀胱进行宏观和组织学评估。根据格雷标准对水肿和出血的存在进行大体评估以及对膀胱损伤进行组织学评估并评分。
对于宏观和微观数据，我们采用Kruskal - Wallis非参数方差分析进行统计评估，随后进行Mann - Whitney U检验。
第二组所有动物均有HC证据。与对照组相比，该组存在明显的组织学损伤和宏观变化（P<0.001）。第三组和第四组膀胱损伤的中位数评分显著低于第二组（P<0.001）。当比较第一组、第三组和第四组膀胱损伤的中位数评分时，这些组之间无显著差异。
本研究证明了氨磷汀在预防环磷酰胺诱导的出血性膀胱炎方面的疗效。
