酿酒酵母糖化酵母编码甘油-3-磷酸脱氢酶的DAR1基因的克隆、测序及破坏。
Cloning, sequence, and disruption of the Saccharomyces diastaticus DAR1 gene encoding a glycerol-3-phosphate dehydrogenase.
作者信息
Wang H T, Rahaim P, Robbins P, Yocum R R
机构信息
OmniGene, Inc., Cambridge, Massachusetts 02139-9002.
出版信息
J Bacteriol. 1994 Nov;176(22):7091-5. doi: 10.1128/jb.176.22.7091-7095.1994.
Abstract
The Saccharomyces diastaticus DAR1 gene was cloned by complementation in an Escherichia coli strain auxogrophic for glycerol-3-phosphate. DAR1 encodes an NADH-dependent dihydroxyacetone phosphate reductase (sn-glycerol-3-phosphate dehydrogenase [G3PDase; EC 1.1.1.8]) homologous to several other eukaryotic G3PDases. DAR1 is distinct from GUT2, which encodes a glucose-repressed mitochondrial G3PDase, but is identical to GPD1 from S. cerevisiae, a close relative of S. diastaticus. The level of DAR1-encoded G3PDase was increased about threefold in a medium of high osmolarity. Disruption of DAR1 in a haploid S. cerevisiae was not lethal but led to a decrease in cytoplasmic NADH-dependent G3PDase activity, an increase in osmotic sensitivity, and a 25% reduction in glycerol secretion from cells grown anaerobically on glucose.
摘要
通过在一株对3-磷酸甘油营养缺陷的大肠杆菌菌株中进行互补，克隆出了糖化酵母（Saccharomyces diastaticus）的DAR1基因。DAR1编码一种依赖NADH的磷酸二羟丙酮还原酶（sn-甘油-3-磷酸脱氢酶[G3PDase；EC 1.1.1.8]），与其他几种真核生物G3PDase同源。DAR1与GUT2不同，GUT2编码一种受葡萄糖抑制的线粒体G3PDase，但与糖化酵母的近亲酿酒酵母（S. cerevisiae）的GPD1相同。在高渗透压培养基中，DAR1编码的G3PDase水平增加了约三倍。单倍体酿酒酵母中DAR1的破坏并不致命，但导致细胞质中依赖NADH的G3PDase活性降低、渗透压敏感性增加，以及在葡萄糖上厌氧生长的细胞甘油分泌减少25%。
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本文引用的文献
1
The sequence of Saccharomyces cerevisiae cloning vector pCS19 allowing direct selection for DNA inserts.酿酒酵母克隆载体pCS19的序列，可直接选择DNA插入片段。
Gene. 1993 Feb 14;124(1):141-2. doi: 10.1016/0378-1119(93)90778-2.
2
GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae.GUT2，酿酒酵母线粒体甘油-3-磷酸脱氢酶的一个基因。
Yeast. 1993 Oct;9(10):1121-30. doi: 10.1002/yea.320091013.
3
Appendix. Cloning and sequence of the gene encoding enzyme E-1 from the methionine salvage pathway of Klebsiella oxytoca.附录。产酸克雷伯菌甲硫氨酸补救途径中编码酶E-1的基因的克隆与序列分析
J Biol Chem. 1993 Nov 25;268(33):24792-5.
4
GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway.GPD1编码3-磷酸甘油脱氢酶，对酿酒酵母在渗透胁迫下的生长至关重要，其表达受高渗甘油反应途径调控。
Mol Cell Biol. 1994 Jun;14(6):4135-44. doi: 10.1128/mcb.14.6.4135-4144.1994.
5
A two-component system that regulates an osmosensing MAP kinase cascade in yeast.一种调节酵母中渗透压感应丝裂原活化蛋白激酶级联反应的双组分系统。
Nature. 1994 May 19;369(6477):242-5. doi: 10.1038/369242a0.
6
A gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae.一个编码sn-甘油-3-磷酸脱氢酶（NAD⁺）的基因可互补酿酒酵母的一个渗透压敏感突变体。
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7
An osmosensing signal transduction pathway in yeast.酵母中的一种渗透感应信号转导途径。
Science. 1993 Mar 19;259(5102):1760-3. doi: 10.1126/science.7681220.
8
Purification and some properties of sn-glycerol-3-phosphate dehydrogenase from Saccharomyces cerevisiae.
Anal Biochem. 1982 May 1;122(1):180-5. doi: 10.1016/0003-2697(82)90268-8.
9
Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.通过酵母中的互补作用分离基因：一个细胞周期基因的分子克隆
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2119-23. doi: 10.1073/pnas.77.4.2119.
10
Yeast growth and glycerol formation.酵母生长与甘油形成。
Acta Chem Scand. 1966;20(4):1016-25. doi: 10.3891/acta.chem.scand.20-1016.
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