Fuxe K, Tinner B, Staines W, Hemsén A, Hersh L, Lundberg J M
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Neurosci Lett. 1991 Feb 11;123(1):107-11. doi: 10.1016/0304-3940(91)90169-t.
Using a rabbit endothelin-1 (ET-1) antiserum together with a goat-anticholineacetylase antiserum or a mouse anti-somatostatin antiserum it was possible by means of double immunolabelling procedures to demonstrate ET-like immunoreactivity in striatal nerve cell bodies of the rat, which were shown to contain either cholineacetylase or somatostatin immunoreactivity. Absorption studies with ET-3, ET-1 or big ET-1 indicated that the ET-like immunoreactivity was ET-3 like. In agreement the radioimmunoassay showed that ET-3-like immunoreactivity was present in higher concentrations than ET-1-like immunoreactivity in the neostriatum and other brain areas. Characterization by reversed phase HPLC revealed, however, that a major portion of the neostriatal ET-3-like immunoreactivity was not identical to ET-3. The nature of neuronal ET in the rat may thus be more complicated than hitherto assumed.
使用兔内皮素-1（ET-1）抗血清与山羊抗胆碱乙酰化酶抗血清或小鼠抗生长抑素抗血清，通过双重免疫标记程序可以在大鼠纹状体神经细胞体中证明ET样免疫反应性，这些细胞体显示含有胆碱乙酰化酶或生长抑素免疫反应性。用ET-3、ET-1或大ET-1进行的吸收研究表明，ET样免疫反应性类似ET-3。同样，放射免疫分析表明，在新纹状体和其他脑区中，ET-3样免疫反应性的浓度高于ET-1样免疫反应性。然而，反相高效液相色谱法表征显示，新纹状体中大部分ET-3样免疫反应性与ET-3并不相同。因此，大鼠神经元ET的性质可能比迄今所认为的更为复杂。
