Tallaksen-Greene S J, Kaatz K W, Romano C, Albin R L
Department of Neurology, University of Michigan, Ann Arbor 48109, USA.
Brain Res. 1998 Jan 12;780(2):210-7. doi: 10.1016/s0006-8993(97)01141-4.
Metabotropic glutamate receptors are important mediators of excitatory amino acid neurotransmission in the striatum. Two-color immunofluorescence histochemistry and immunohistochemistry in combination with retrograde tract-tracing techniques were used to examine the distribution of metabotropic glutamate receptor subtypes 1a and 5 (mGluR1a and mGluR5) among identified subpopulations of striatal projection neurons and interneurons. The majority of striatopallidal and striatonigral neurons were double-labeled for both mGluR1a or mGluR5. Approximately 60% to 70% of either striatonigral or striatopallidal neurons expressed mGluR1a- or mGluR5-like immunoreactivity. The percentage of double-labeled striatopallidal or striatonigral projection neurons did not differ among striatal quadrants. Striatal interneurons expressing parvalbumin or somatostatin or choline acetyltransferase exhibited varying degrees of expression of mGluR1a or mGluR5. Virtually all (94%) parvalbumin-immunoreactive striatal neurons expressed mGluR1a-like immunoreactivity with a majority (79%) of these neurons expressing mGluR5-like immunoreactivity. A high percentage (89%) of striatal choline acetyltransferase-immunoreactive neurons were double-labeled for mGluR1a-like immunoreactivity. Approximately 65% of striatal choline acetyltransferase-immunoreactive neurons expressed mGluR5-like immunoreactivity. A majority (65%) of somatostatin-immunoreactive striatal interneurons expressed mGluR1a-like immunoreactivity with a slightly lower percentage (55%) expressing mGluR5-like immunoreactivity. These findings indicate considerable heterogeneity among striatal projection and interneurons with respect to mGluR1a and mGluR5 expression. There may be subpopulations of striatonigral and striatopallidal projection neurons. These results are consistent as well with prior data indicating subpopulations of the different classes of striatal interneurons.
代谢型谷氨酸受体是纹状体中兴奋性氨基酸神经传递的重要介质。采用双色免疫荧光组织化学和免疫组织化学结合逆行束路追踪技术，研究代谢型谷氨酸受体1a和5亚型（mGluR1a和mGluR5）在纹状体投射神经元和中间神经元特定亚群中的分布。大多数纹状体苍白球和纹状体黑质神经元对mGluR1a或mGluR5均呈双标记。约60%至70%的纹状体黑质或纹状体苍白球神经元表达mGluR1a样或mGluR5样免疫反应性。双标记的纹状体苍白球或纹状体黑质投射神经元的百分比在纹状体象限之间没有差异。表达小白蛋白、生长抑素或胆碱乙酰转移酶的纹状体中间神经元表现出不同程度的mGluR1a或mGluR5表达。几乎所有（94%）小白蛋白免疫反应性纹状体神经元都表达mGluR1a样免疫反应性，其中大多数（79%）神经元表达mGluR5样免疫反应性。高比例（89%）的纹状体胆碱乙酰转移酶免疫反应性神经元对mGluR1a样免疫反应性呈双标记。约65%的纹状体胆碱乙酰转移酶免疫反应性神经元表达mGluR5样免疫反应性。大多数（65%）生长抑素免疫反应性纹状体中间神经元表达mGluR1a样免疫反应性，表达mGluR5样免疫反应性的百分比略低（55%）。这些发现表明，在mGluR1a和mGluR5表达方面，纹状体投射神经元和中间神经元之间存在相当大的异质性。可能存在纹状体黑质和纹状体苍白球投射神经元的亚群。这些结果也与先前表明不同类型纹状体中间神经元亚群的数据一致。
