Neely Alice N, Regnold Lisa A, Hayes Susan, Gardner Jason, Cahill Thomas J
Department of Infection Control, University of Cincinnati College of Medicine, USA.
Am J Infect Control. 2006 Jun;34(5):281-4. doi: 10.1016/j.ajic.2005.12.014.
Patients with tracheostomies carry a case for suctioning supplies. A returned case cultured positive for Staphylococcus aureus even after it had been cleaned with a disinfectant. The purposes of this study were to determine (1) the source of the S aureus and (2) how to provide a microbiologically safe case for the next patient.
Reviews of patient and environmental cultures plus pulsed-field gel electrophoresis (PFGE) were used to determine the source of the S aureus. Repeated wiping with various detergents/disinfectants did not remove the S aureus from the case, which could not be immersed for cleaning because of a chipboard component. We designed a case made of denim with a washable synthetic fiber to provide shape. The new cases were purposefully contaminated and laundered and then cultured.
PFGE indicated that the S aureus was from the patient who had most recently used the case. Contamination of the denim case with the original S aureus, followed by laundering, resulted in a case that was free of the S aureus. These results were repeated for other pathogens.
Commercially available cases for suction equipment can become contaminated with pathogens from the user and may be difficult to disinfect. By making the case out of washable materials, less expensive cases, which could be readily disinfected, resulted.
气管切开术患者会携带吸痰用品盒。一个归还的用品盒即使在用消毒剂清洁后，培养出金黄色葡萄球菌呈阳性。本研究的目的是确定（1）金黄色葡萄球菌的来源，以及（2）如何为下一位患者提供一个微生物学安全的用品盒。
通过对患者和环境培养物的回顾以及脉冲场凝胶电泳（PFGE）来确定金黄色葡萄球菌的来源。用各种洗涤剂/消毒剂反复擦拭未能从用品盒中清除金黄色葡萄球菌，由于有硬纸板部件，该用品盒不能浸泡清洗。我们设计了一种由牛仔布制成、带有可清洗合成纤维以保持形状的用品盒。将新的用品盒故意污染后进行清洗，然后进行培养。
PFGE表明金黄色葡萄球菌来自最近使用该用品盒的患者。牛仔布用品盒被原来的金黄色葡萄球菌污染，随后进行清洗，结果用品盒中没有了金黄色葡萄球菌。其他病原体也得到了同样的结果。
市售的吸痰设备用品盒可能会被使用者的病原体污染，且可能难以消毒。通过用可清洗材料制作用品盒，可以得到更便宜且易于消毒的用品盒。
