Pannetier Maëlle, Tilly Gaëlle, Kocer Ayhan, Hudrisier Marthe, Renault Lauriane, Chesnais Nathalie, Costa José, Le Provost Fabienne, Vaiman Daniel, Vilotte Jean-Luc, Pailhoux Eric
INRA, Biologie du Développement et Reproduction, Bât. J. Poly, 78350 Jouy-en-Josas, France.
FEBS Lett. 2006 Jun 26;580(15):3715-20. doi: 10.1016/j.febslet.2006.05.060.
The testis-determining gene SRY is not well-conserved among mammals, particularly between mouse and other mammals, both in terms of protein structure and of expression regulation. To evaluate SRY phylogenic conservation in regards to its function, we expressed the goat gene (gSRY) in XX transgenic mouse gonads. Here, we show that gSRY induces testis formation, despite a goat expression profile. Our results demonstrate that sex-reversal can be induced in XX-mice by a non-mouse SRY thus suggesting a conserved molecular mechanism of action of this testis-determining gene across mammalian species.
睾丸决定基因SRY在哺乳动物中，尤其是在小鼠和其他哺乳动物之间，无论在蛋白质结构还是表达调控方面，都没有得到很好的保守。为了评估SRY在其功能方面的系统发育保守性，我们在XX转基因小鼠性腺中表达了山羊基因（gSRY）。在这里，我们表明，尽管具有山羊的表达谱，gSRY仍能诱导睾丸形成。我们的结果表明，非小鼠的SRY可以在XX小鼠中诱导性反转，从而提示该睾丸决定基因在哺乳动物物种间作用的分子机制具有保守性。
