Sheng Guo-Ping, Yu Han-Qing, Wang Cheng-Ming
Laboratory of Environmental Biotechnology, School of Chemistry, University of Science and Technology of China, Hefei, 230026, People's Republic of China.
Appl Microbiol Biotechnol. 2006 Nov;73(1):204-10. doi: 10.1007/s00253-006-0442-2. Epub 2006 Jun 10.
The Fourier transform infrared (FTIR) spectra of the cells of two photosynthetic H(2)-producing strains, Rhodoblastus acidophilus and Rhodobacter capsulatus, as well as their extracellular polymeric substances (EPS), were evaluated. The FTIR spectra of R. capsulatus and its EPS during its cultivation were also recorded. The main peaks in the spectra, including 1,080 cm(-1) (carbohydrates), 1,250 cm(-1) (nucleic acids), 2,830-2,930 cm(-1) (lipids), 1,660-1,535 cm(-1) (Amide I and II of proteins), were observed. The relative heights of these peaks in the spectra of the two strains were different, showing the difference in contents of various components in the cells or EPS. The ratios among the main components in the EPS obtained from the FTIR spectra were in good agreement with those from a conventional quantitative chemical analysis. As an easy, rapid, and direct technique, the FTIR spectroscopy could be used to characterize the components and their relative contents of EPS of photosynthetic bacteria.
对两株光合产氢菌株嗜酸红假单胞菌和荚膜红细菌的细胞及其胞外聚合物（EPS）的傅里叶变换红外（FTIR）光谱进行了评估。还记录了荚膜红细菌及其EPS在培养过程中的FTIR光谱。观察到光谱中的主要峰，包括1080 cm⁻¹（碳水化合物）、1250 cm⁻¹（核酸）、2830 - 2930 cm⁻¹（脂质）、1660 - 1535 cm⁻¹（蛋白质的酰胺I和II）。这两株菌株光谱中这些峰的相对高度不同，表明细胞或EPS中各种成分的含量存在差异。从FTIR光谱获得的EPS中主要成分的比例与传统定量化学分析得到的比例高度一致。作为一种简单、快速且直接的技术，FTIR光谱可用于表征光合细菌EPS的成分及其相对含量。
