Song Xing-bo, Fan Hong, Ying Bin-wu, Lu Xiao-jun, Wang Jun, Ye Yuan-xin
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Oct;29(10):1973-6.
To obtain the population genetic data of 17 Y-chromosomal short tandem repeat (Y-STR) in the Han population in Chengdu of Sichuan Province.
The 17 Y-STR loci were amplified from the blood samples of 111 unrelated Chengdu Han individuals using the AmpFlSTR Yfiler system. The PCR products were genotyped with an ABI 3130 genetic analyzer.
In the loci of in DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438, and DYS448, 3 to 8 alleles were detected in the Han population in Chengdu, and 36 alleles were detected in the locus DYS385a/b, with the minimal gene diversity (GD) value of 0.3970 (DYS391) and maximal value of 0.9561 (DYS385a/b). The DNA samples of 16 women and 7 different species of animals were amplified, but no specific products were found for the 17 Y-STR loci. No mutations of the 17 Y-STR alleles were observed in 20 father-son pairs as confirmed by autosomal STR analysis.
The 17 Y-STR loci are highly polymorphic and are suitable for personal identification, paternity testing, population genetics and anthropology studies.
获取四川省成都市汉族人群17个Y染色体短串联重复序列（Y-STR）的群体遗传学数据。
采用AmpFlSTR Yfiler系统，从111名成都汉族无关个体的血液样本中扩增17个Y-STR基因座。PCR产物用ABI 3130遗传分析仪进行基因分型。
在DYS456、DYS389I、DYS390、DYS389II、DYS458、DYS19、DYS385a/b、DYS393、DYS391、DYS439、DYS635、DYS392、Y-GATA-H4、DYS437、DYS438和DYS448基因座中，成都汉族人群检测到3至8个等位基因，DYS385a/b基因座检测到36个等位基因，最小基因多样性（GD）值为0.3970（DYS391），最大为0.9561（DYS385a/b）。对16名女性和7种不同动物的DNA样本进行扩增，未发现17个Y-STR基因座的特异性产物。经常染色体STR分析证实，20对父子的17个Y-STR等位基因均未发生突变。
17个Y-STR基因座具有高度多态性，适用于个人识别、亲子鉴定、群体遗传学和人类学研究。
